Jeongbin Park1, Sangsu Bae2, Jin-Soo Kim3. 1. Department of Chemistry. 2. Department of Chemistry, Institute for Materials Design, Hanyang University, Seoul, South Korea. 3. Center for Genome Engineering, Institute for Basic Science, Seoul, South Korea and Department of Chemistry, Seoul National University, Seoul, South Korea.
Abstract
UNLABELLED: We present Cas-Designer, a user-friendly program to aid researchers in choosing appropriate target sites in a gene of interest for type II CRISPR/Cas-derived RNA-guided endonucleases, which are now widely used for biomedical research and biotechnology. Cas-Designer rapidly provides the list of all possible guide RNA sequences in a given input DNA sequence and their potential off-target sites including bulge-type sites in a genome of choice. In addition, the program assigns an out-of-frame score to each target site to help users choose appropriate sites for gene knockout. Cas-Designer shows the results in an interactive table and provides user-friendly filter functions. AVAILABILITY AND IMPLEMENTATION: Free access at http://rgenome.net/cas-designer/.
UNLABELLED: We present Cas-Designer, a user-friendly program to aid researchers in choosing appropriate target sites in a gene of interest for type II CRISPR/Cas-derived RNA-guided endonucleases, which are now widely used for biomedical research and biotechnology. Cas-Designer rapidly provides the list of all possible guide RNA sequences in a given input DNA sequence and their potential off-target sites including bulge-type sites in a genome of choice. In addition, the program assigns an out-of-frame score to each target site to help users choose appropriate sites for gene knockout. Cas-Designer shows the results in an interactive table and provides user-friendly filter functions. AVAILABILITY AND IMPLEMENTATION: Free access at http://rgenome.net/cas-designer/.