Johanna Maukonen1, Kaija-Leena Kolho2, Monika Paasela3, Jarno Honkanen2, Paula Klemetti2, Outi Vaarala4, Maria Saarela5. 1. VTT Technical Research Centre of Finland, Espoo, Finland johanna.maukonen@vtt.fi. 2. Hospital for Children and Adolescents, University of Helsinki, Helsinki, Finland. 3. National Institute for Health and Welfare [THL], Helsinki, Finland. 4. Hospital for Children and Adolescents, University of Helsinki, Helsinki, Finland National Institute for Health and Welfare [THL], Helsinki, Finland Respiratory, Inflammatory and Autoimmune Diseases, Innovative Medicine, AstraZeneca, Mölndal, Sweden. 5. VTT Technical Research Centre of Finland, Espoo, Finland.
Abstract
BACKGROUND AND AIMS: Several factors support the view of inflammatory bowel disease [IBD] origin in the host responsiveness to intestinal bacteria, although no single bacterial species has been shown as a causative agent in the pathogenesis. Our aim was to analyse the fecal microbiota of paediatric IBD patients at different stages of the disease. In addition, the characteristics of immune response to the bacterial isolates showing very low abundance in IBD were studied. METHODS: Fecal samples [1-3 samples/child] were collected from 10 paediatric patients with crohn's disease [CD], and 12 with ulcerative colitis [UC] and from 8 healthy children, for polyphasic microbiological analysis (culture, real-time polymerase chain reaction [PCR], and denaturing gradient gel electrophoresis). In addition, in vitro cytokine responses of peripheral blood mononuclear cells to the bacterial isolates, which showed very low abundance in IBD, were studied. RESULTS: Although predominant bacterial diversity was higher in IBD, the numbers of Lachnospiraceae and Coriobacteriaceae bacteria were lower in IBD patients as compared with control children [p < 0.05]. In addition, Ruminococcaceae population diversity was lower in IBD [p < 0.05] and correlated negatively with fecal calprotectin levels. Both abundance and diversity of bifidobacterial populations were lower in children with IBD [p < 0.05], and particularly low numbers of certain bifidobacterial isolates were detected. In CD, we found enhanced up-regulation of interleukin-6 transcripts and impaired RAR-related orphan receptor C response to bifidobacteria, whereas decreased interferon-gamma response was observed in both CD and UC. CONCLUSION: We demonstrate altered fecal microbiota in paediatric IBD, particularly low numbers and diversity of bifidobacterial populations. Interestingly, immunological response to bifidobacteria differed between paediatric CD patients and control children.
BACKGROUND AND AIMS: Several factors support the view of inflammatory bowel disease [IBD] origin in the host responsiveness to intestinal bacteria, although no single bacterial species has been shown as a causative agent in the pathogenesis. Our aim was to analyse the fecal microbiota of paediatric IBD patients at different stages of the disease. In addition, the characteristics of immune response to the bacterial isolates showing very low abundance in IBD were studied. METHODS: Fecal samples [1-3 samples/child] were collected from 10 paediatric patients with crohn's disease [CD], and 12 with ulcerative colitis [UC] and from 8 healthy children, for polyphasic microbiological analysis (culture, real-time polymerase chain reaction [PCR], and denaturing gradient gel electrophoresis). In addition, in vitro cytokine responses of peripheral blood mononuclear cells to the bacterial isolates, which showed very low abundance in IBD, were studied. RESULTS: Although predominant bacterial diversity was higher in IBD, the numbers of Lachnospiraceae and Coriobacteriaceae bacteria were lower in IBD patients as compared with control children [p < 0.05]. In addition, Ruminococcaceae population diversity was lower in IBD [p < 0.05] and correlated negatively with fecal calprotectin levels. Both abundance and diversity of bifidobacterial populations were lower in children with IBD [p < 0.05], and particularly low numbers of certain bifidobacterial isolates were detected. In CD, we found enhanced up-regulation of interleukin-6 transcripts and impaired RAR-related orphan receptor C response to bifidobacteria, whereas decreased interferon-gamma response was observed in both CD and UC. CONCLUSION: We demonstrate altered fecal microbiota in paediatric IBD, particularly low numbers and diversity of bifidobacterial populations. Interestingly, immunological response to bifidobacteria differed between paediatric CDpatients and control children.
Authors: Marc Jan Bonder; Ettje F Tigchelaar; Xianghang Cai; Gosia Trynka; Maria C Cenit; Barbara Hrdlickova; Huanzi Zhong; Tommi Vatanen; Dirk Gevers; Cisca Wijmenga; Yang Wang; Alexandra Zhernakova Journal: Genome Med Date: 2016-04-21 Impact factor: 11.117
Authors: Rongsong Li; Jieping Yang; Arian Saffari; Jonathan Jacobs; Kyung In Baek; Greg Hough; Muriel H Larauche; Jianguo Ma; Nelson Jen; Nabila Moussaoui; Bill Zhou; Hanul Kang; Srinivasa Reddy; Susanne M Henning; Matthew J Campen; Joseph Pisegna; Zhaoping Li; Alan M Fogelman; Constantinos Sioutas; Mohamad Navab; Tzung K Hsiai Journal: Sci Rep Date: 2017-02-17 Impact factor: 4.379
Authors: K R Hughes; L C Harnisch; C Alcon-Giner; S Mitra; C J Wright; J Ketskemety; D van Sinderen; A J M Watson; L J Hall Journal: Open Biol Date: 2017-01 Impact factor: 6.411