Daniel F Martins1, Aline A Emer2, A P Batisti3, Nathalia Donatello3, Mariana G Carlesso3, Leidiane Mazzardo-Martins4, Dalila Venzke5, Gustavo A Micke5, Moacir G Pizzolatti5, A P Piovezan2, A R S dos Santos6. 1. Experimental Neuroscience Laboratory (LaNEx), University of Southern Santa Catarina at Palhoça, Santa Catarina, Brazil; Postgraduate Program in Health Sciences, University of Southern Santa Catarina at Palhoça, Santa Catarina, Brazil. Electronic address: daniel.martins4@unisul.br. 2. Experimental Neuroscience Laboratory (LaNEx), University of Southern Santa Catarina at Palhoça, Santa Catarina, Brazil; Postgraduate Program in Health Sciences, University of Southern Santa Catarina at Palhoça, Santa Catarina, Brazil. 3. Experimental Neuroscience Laboratory (LaNEx), University of Southern Santa Catarina at Palhoça, Santa Catarina, Brazil. 4. Department of Morphological Sciences, Centre of Biological Sciences, University Federal of Santa Catarina, Florianópolis, Santa Catarina, Brazil. 5. Department of Chemistry, University Federal of Santa Catarina, Florianópolis, Santa Catarina, Brazil. 6. Laboratory of Neurobiology of Pain and Inflammation, Department of Physiological Sciences, Centre of Biological Sciences, University Federal of Santa Catarina, Florianópolis, Santa Catarina, Brazil.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Cedrus atlantica essential oil (CaEO) presents analgesic and anti-inflammatory sedative properties. However, it remains unknown whether CaEO alleviates acute postoperative pain. MATERIALS AND METHODS: Here, we investigated the effect of CaEO on postoperative pain and its mechanisms related to the descending pain control in Swiss males mice induced by a plantar incision surgery (PIS) in the hindpaw. RESULTS: Inhalation of CaEO (5', 30' or 60') markedly reduced mechanical hypersensitivity. This effect was prevented by pre-treatment with naloxone or p-chlorophenylalanine methyl ester (PCPA, 100mg/kg, i.p.)-induced depletion of serotonin. In addition, p-alpha-methyl-para-tyrosin (AMPT, 100mg/kg, i.p.)-induced depletion of norepinephrine, intraperitoneal injection of the α2-adrenergic receptor antagonist yohimbine (0.15 mg/kg, i.p.) or haloperidol (1mg/kg, i.p.) an antagonist of dopaminergic (D1 and D2) receptors prevented the effect of CaEO on hypersensitivity. CONCLUSIONS: These findings suggest that CaEO alleviates postoperative pain by activating the descending pain modulation pathways on the opioidergic, serotonergic, noradrenergic (α2-adrenergic) and dopaminergic (dopamine D1 and D2 receptors) systems.
ETHNOPHARMACOLOGICAL RELEVANCE: Cedrus atlantica essential oil (CaEO) presents analgesic and anti-inflammatory sedative properties. However, it remains unknown whether CaEO alleviates acute postoperative pain. MATERIALS AND METHODS: Here, we investigated the effect of CaEO on postoperative pain and its mechanisms related to the descending pain control in Swiss males mice induced by a plantar incision surgery (PIS) in the hindpaw. RESULTS: Inhalation of CaEO (5', 30' or 60') markedly reduced mechanical hypersensitivity. This effect was prevented by pre-treatment with naloxone or p-chlorophenylalanine methyl ester (PCPA, 100mg/kg, i.p.)-induced depletion of serotonin. In addition, p-alpha-methyl-para-tyrosin (AMPT, 100mg/kg, i.p.)-induced depletion of norepinephrine, intraperitoneal injection of the α2-adrenergic receptor antagonist yohimbine (0.15 mg/kg, i.p.) or haloperidol (1mg/kg, i.p.) an antagonist of dopaminergic (D1 and D2) receptors prevented the effect of CaEO on hypersensitivity. CONCLUSIONS: These findings suggest that CaEO alleviates postoperative pain by activating the descending pain modulation pathways on the opioidergic, serotonergic, noradrenergic (α2-adrenergic) and dopaminergic (dopamine D1 and D2 receptors) systems.
Authors: Rafael Pazinato; Andréia Volpato; Matheus D Baldissera; Roberto C V Santos; Dilmar Baretta; Rodrigo A Vaucher; Janice L Giongo; Aline A Boligon; Lenita Moura Stefani; Aleksandro Schafer Da Silva Journal: J Adv Res Date: 2016-05-11 Impact factor: 10.479