Parisa Kolahdouz1, Ehsan Farashahi Yazd2,3, Masoud Tajamolian1, Masoud Reza Manaviat4,5, Mohammad Hasan Sheikhha1,6. 1. Department of Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences Campus, Shohadaye Gomnam Blvd., 8915173143, Yazd, Iran. 2. Department of Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences Campus, Shohadaye Gomnam Blvd., 8915173143, Yazd, Iran. ehsanfarashahi@gmail.com. 3. Yazd Clinical & Research Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. ehsanfarashahi@gmail.com. 4. Yazd Diabetes Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 5. Department of Ophthalmology, School of Medicine, Shahid University of Medical Sciences, Yazd, Iran. 6. Yazd Clinical & Research Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Abstract
PURPOSE: This study aims at investigating possible associations of P-selectin polymorphisms with proliferative diabetic retinopathy (PDR) in Yazd, Iran. METHODS: The subjects of the study included of 55 PDR and 55 diabetic no retinopathy (DNR) cases attending Yazd Diabetes Research Center (YDRC). P-selectin genotyping was done by an ARMS-PCR method. RESULTS: The P-selectin variants rs6128, rs6133, and rs3917779 were not in Hardy-Weinberg equilibrium. The frequency of the rs3917779 C allele (P < 0.0001), but not the rs6133 G allele (P = 0.19) or rs6128 allele (P = 0.20), was higher in PDR cases than in control DNR cases. Significant differences in the distribution of rs3917779 (P < 0.001), but not rs6128 (P = 0.52) or rs6133 (P = 0.18), genotypes were observed between cases and controls, and only rs3917779 showed a significant association with PDR, with increments of 49.2 (14.72-125.07) in disease risk seen for CC genotypes. Among the eight three-locus P-selectin haplotypes constructed (rs6128 / rs6133 / rs3917779), there was no significant difference between frequencies of haplotypes in the DNR and PDR groups. CONCLUSIONS: P-selectin gene polymorphisms and haplotypes can contribute to PDR development.
PURPOSE: This study aims at investigating possible associations of P-selectin polymorphisms with proliferative diabetic retinopathy (PDR) in Yazd, Iran. METHODS: The subjects of the study included of 55 PDR and 55 diabetic no retinopathy (DNR) cases attending Yazd Diabetes Research Center (YDRC). P-selectin genotyping was done by an ARMS-PCR method. RESULTS: The P-selectin variants rs6128, rs6133, and rs3917779 were not in Hardy-Weinberg equilibrium. The frequency of the rs3917779 C allele (P < 0.0001), but not the rs6133 G allele (P = 0.19) or rs6128 allele (P = 0.20), was higher in PDR cases than in control DNR cases. Significant differences in the distribution of rs3917779 (P < 0.001), but not rs6128 (P = 0.52) or rs6133 (P = 0.18), genotypes were observed between cases and controls, and only rs3917779 showed a significant association with PDR, with increments of 49.2 (14.72-125.07) in disease risk seen for CC genotypes. Among the eight three-locus P-selectin haplotypes constructed (rs6128 / rs6133 / rs3917779), there was no significant difference between frequencies of haplotypes in the DNR and PDR groups. CONCLUSIONS:P-selectin gene polymorphisms and haplotypes can contribute to PDR development.
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