| Literature DB >> 26342791 |
Rafael Pereira Heckler1, Dyego Gonçalves Lino Borges1, Flávia Barbieri Bacha1, Marcel Kenzo Vilalba Onizuka1, Letícia e Silva Teruya1, Juliana Paniago Lordelo Neves1, Cássia Rejane Brito Leal1, Ricardo Antônio Amaral de Lemos1, Marcelo Vasconcelos Meireles2, Fernando de Almeida Borges3.
Abstract
The presence of Cryptosporidium spp. in a cattle herd registered with an outbreak of diarrhea was investigated and the the molecular subtyping of Cryptosporidium parvum was characterized. Fecal samples from 85 Nellore beef cattle (Bos indicus) were collected and examined with Ziehl-Neelsen modified staining method. Fifty-four cattle (63.52%) had Cryptosporidium spp. oocysts in their feces. Fragments of genes encoding the 18S ribosomal RNA subunit and a 60-kDa glycoprotein (gp60) were amplified by nested PCR accomplished in the 11 most heavily parasitized samples, and the amplicons were sequenced. Eight of the 11 analyzed samples were positive for 18S rRNA sequences and identified monospecific infections with C. parvum. Seven samples were positive for gp60 and identified subtypes IIaA15G2R1 (6/11) and IIaA14G2R1 (1/11). This report is the first for C. parvum subtype IIaA14G2R1 in beef cattle in Brazil.Entities:
Keywords: Cryptosporidiosis; Diarrhea; IIaA14G2R1; IIaA15G2R1; Oocysts; gp60
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Year: 2015 PMID： 26342791 DOI： 10.1016/j.prevetmed.2015.08.016
Source DB: PubMed Journal: Prev Vet Med ISSN： 0167-5877 Impact factor: 2.670