Noura Ramadan Abdel-Hamid1, Eman A Mohammed2, Ashraf H Abbas3, Fouad M Badr4. 1. Genetics Unit, Department of Histology and Cell Biology, Faculty of Medicine, Suez Canal University, Circular Road, Ismailia, 41522, Egypt. nour_genetics@yahoo.com. 2. Genetics Unit, Department of Histology and Cell Biology, Faculty of Medicine, Suez Canal University, Circular Road, Ismailia, 41522, Egypt. e_moemen@hotmail.com. 3. Department of Plastic Surgery, Faculty of Medicine, Suez Canal University, Ismailia, Egypt. dr_ashrafhussein@yahoo.com. 4. Genetics Unit, Department of Histology and Cell Biology, Faculty of Medicine, Suez Canal University, Circular Road, Ismailia, 41522, Egypt. fmbadr@hotmail.com.
Abstract
INTRODUCTION: Breast cancer (BC) is the most common cancer amongst Egyptian women and worldwide. MicroRNA-21 (miR-21), on chromosome 17q21.3, is one of the most up-regulated microRNAs in cancer that silences multiple target genes involved in cancer-signaling pathways. The aim of this study was to assess the correlation between numerical aberrations of chromosome 17 and the miR-21 expression profile in BC tissues in female Egyptian patients. METHODS: The study included 37 female patients with sporadic primary breast carcinoma, their age ranged from 31 to 65 years. Fresh breast tissue specimens were evaluated for miR-21 expression levels using reverse transcription-polymerase chain reaction technology and cytogenetic fluorescent in situ hybridization analysis for chromosome 17 aneusomy. RESULTS: miR-21 was up-regulated 12.9-fold in BC tissues compared with normal adjacent tissue. Over-expression was significantly associated with several clinico-pathologic characteristics; as higher tumor grade, more tumor size, advanced stage, and poor prognostic index. In addition, chromosome 17 monosomy and trisomy were observed in 21.6 and 5.4 % of BC patients, respectively. However, the large majority (73 %) of patients had heterogeneous cell populations. Chromosome 17 copy number heterogeneity in cell populations were significantly associated with advanced clinical stage and higher miR-21 expression profile in BC tissues. CONCLUSION: Chromosome 17 aneusomy and miR-21 expression are positively correlated and can potentially serve as prognostic markers in BC.
INTRODUCTION:Breast cancer (BC) is the most common cancer amongst Egyptian women and worldwide. MicroRNA-21 (miR-21), on chromosome 17q21.3, is one of the most up-regulated microRNAs in cancer that silences multiple target genes involved in cancer-signaling pathways. The aim of this study was to assess the correlation between numerical aberrations of chromosome 17 and the miR-21 expression profile in BC tissues in female Egyptian patients. METHODS: The study included 37 female patients with sporadic primary breast carcinoma, their age ranged from 31 to 65 years. Fresh breast tissue specimens were evaluated for miR-21 expression levels using reverse transcription-polymerase chain reaction technology and cytogenetic fluorescent in situ hybridization analysis for chromosome 17 aneusomy. RESULTS:miR-21 was up-regulated 12.9-fold in BC tissues compared with normal adjacent tissue. Over-expression was significantly associated with several clinico-pathologic characteristics; as higher tumor grade, more tumor size, advanced stage, and poor prognostic index. In addition, chromosome 17 monosomy and trisomy were observed in 21.6 and 5.4 % of BC patients, respectively. However, the large majority (73 %) of patients had heterogeneous cell populations. Chromosome 17 copy number heterogeneity in cell populations were significantly associated with advanced clinical stage and higher miR-21 expression profile in BC tissues. CONCLUSION: Chromosome 17 aneusomy and miR-21 expression are positively correlated and can potentially serve as prognostic markers in BC.
Authors: Mohamed M Hafez; Zeinab K Hassan; Abdel Rahman N Zekri; Ayman A Gaber; Salem S Al Rejaie; Mohamed M Sayed-Ahmed; Othman Al Shabanah Journal: Asian Pac J Cancer Prev Date: 2012
Authors: J Zhang; H Jin; H Liu; S Lv; B Wang; R Wang; H Liu; M Ding; Y Yang; L Li; J Zhang; S Fu; D Xie; M Wu; W Zhou; Q Qian Journal: Oncogenesis Date: 2014-04-14 Impact factor: 7.485