| Literature DB >> 2634248 |
U Förstermann1, K Ishii, L D Gorsky, F Murad.
Abstract
The cytosolic fraction of N1E-115 neuroblastoma cells catalysed the L-arginine- and NADPH-dependent formation of a substance that relaxed endothelium-denuded strips of rabbit aorta. Relaxations in response to this substance were enhanced in the presence of superoxide dismutase. N omega-Nitro-L-arginine and NG-monomethyl-L-arginine, two inhibitors of EDRF synthesis, markedly attenuated the relaxations. Hemoglobin, a scavenger of EDRF, and methylene blue, an inhibitor of soluble guanylate cyclase, completely abolished the relaxation to N1E-115 cytosol. In contrast, the cyclo-oxygenase inhibitor indomethacin did not alter the relaxations. These data demonstrate that the cytosol of a neuronally-derived cell line is able to synthesize a substance with pharmacological properties similar to EDRF.Entities:
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Year: 1989 PMID： 2634248 DOI： 10.1007/bf00169689
Source DB: PubMed Journal: Naunyn Schmiedebergs Arch Pharmacol ISSN： 0028-1298 Impact factor: 3.000