Literature DB >> 26341612

An improved Tn7-lux reporter for broad host range, chromosomally-integrated promoter fusions in Gram-negative bacteria.

Angela Glassing1, Thomas A Lewis2.   

Abstract

An improved vector for chromosomally-integrated promoter-lux fusions is described. The modified vector was tested in parallel with the unmodified vector using the well-characterized Escherichia coli araBAD promoter in the Pseudomonas aeruginosa attTn7 site. The modified mini-Tn7 showed reduced background luminescence, and increased luminescence upon induction, giving >16-fold higher induction ratio.
Copyright © 2015 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Luciferase; Reporter; Tn7; araBAD; lux

Mesh：

Substances：

Year:  2015        PMID： 26341612      PMCID： PMC4624500          DOI： 10.1016/j.mimet.2015.08.016

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  9 in total

1.  Construction of a Tn7-lux system for gene expression studies in gram-negative bacteria.

Authors:  H Shen; S E Gold; S J Tamaki; N T Keen
Journal:  Gene       Date:  1992-12-01       Impact factor: 3.688

2.  Construction of a broad-host-range Tn7-based vector for single-copy P(BAD)-controlled gene expression in gram-negative bacteria.

Authors:  F Heath Damron; Elizabeth S McKenney; Herbert P Schweizer; Joanna B Goldberg
Journal:  Appl Environ Microbiol       Date:  2012-11-02       Impact factor: 4.792

3.  Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis.

Authors:  F Heath Damron; Elizabeth S McKenney; Mariette Barbier; George W Liechti; Herbert P Schweizer; Joanna B Goldberg
Journal:  Appl Environ Microbiol       Date:  2013-04-12       Impact factor: 4.792

Review 4.  Bacterial bioluminescence: organization, regulation, and application of the lux genes.

Authors:  E A Meighen
Journal:  FASEB J       Date:  1993-08       Impact factor: 5.191

5.  mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa.

Authors:  Kyoung-Hee Choi; Herbert P Schweizer
Journal:  Nat Protoc       Date:  2006       Impact factor: 13.491

6.  Role for ferredoxin:NAD(P)H oxidoreductase (FprA) in sulfate assimilation and siderophore biosynthesis in Pseudomonads.

Authors:  Thomas A Lewis; Angela Glassing; Justin Harper; Michael J Franklin
Journal:  J Bacteriol       Date:  2013-06-21       Impact factor: 3.490

7.  A panel of Tn7-based vectors for insertion of the gfp marker gene or for delivery of cloned DNA into Gram-negative bacteria at a neutral chromosomal site.

Authors:  B Koch; L E Jensen; O Nybroe
Journal:  J Microbiol Methods       Date:  2001-07       Impact factor: 2.363

8.  Broad-host-range expression vectors that carry the L-arabinose-inducible Escherichia coli araBAD promoter and the araC regulator.

Authors:  J R Newman; C Fuqua
Journal:  Gene       Date:  1999-02-18       Impact factor: 3.688

9.  Chromosome position effects on gene expression in Escherichia coli K-12.

Authors:  Jack A Bryant; Laura E Sellars; Stephen J W Busby; David J Lee
Journal:  Nucleic Acids Res       Date:  2014-09-10       Impact factor: 16.971
  9 in total

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