| Literature DB >> 26341471 |
Xin Huang1, Pei Yang2, Zhao Yang3, Hong Zhang4, Aiqun Ma5.
Abstract
The expression of hyperpolarization-activated cyclic nucleotide-gated cation (HCN) channel isoforms varies among species, cardiac tissues, developmental stages, and disease generation. However, alterations in the HCN channels during aging remain unclear. We investigated the protein expressions of HCN channel isoforms, HCN1-HCN4, in the sinoatrial nodes (SANs) from young (1-month-old), adult (4-month-old), and aged (30-month-old) rats. We found that HCN2 and HCN4 proteins were present in rat SAN using immunohistochemistry; therefore, we quantitatively analyzed their expression by Western blot. Aim to correlate protein expression and pacemaking function, specific blockade of HCN channels with 3 µmol/L ivabradine prolonged the cycle length in the intact rat heart. During the senescent process, the HCN2 and HCN4 protein levels declined, which was accompanied with a decreased effect of ivabradine on rat SAN automaticity. These results indicated the age-associated expression and relative function of HCN channel isoforms.Entities:
Keywords: Aging; ion channels; sick sinus syndrome; sinoatrial node
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Year: 2015 PMID： 26341471 PMCID： PMC4935443 DOI： 10.1177/1535370215603515
Source DB: PubMed Journal: Exp Biol Med (Maywood) ISSN： 1535-3699