Karina Yuri Kondo1, Marília Afonso Rabelo Buzalaf2, Michele Mauricio Manarelli1, Alberto Carlos Botazzo Delbem1, Juliano Pelim Pessan3. 1. Department of Pediatric Dentistry and Public Health, Araçatuba Dental School, Univ. Estadual Paulista (UNESP), Rua José Bonifácio 1193, CEP: 16015-050, Araçatuba, SP, Brazil. 2. Bauru Dental School, University of São Paulo, Bauru, SP, Brazil. 3. Department of Pediatric Dentistry and Public Health, Araçatuba Dental School, Univ. Estadual Paulista (UNESP), Rua José Bonifácio 1193, CEP: 16015-050, Araçatuba, SP, Brazil. jpessan@foa.unesp.br.
Abstract
INTRODUCTION:Acidic dentifrices have been shown to be more effective than neutral ones against dental caries using in vitro, in situ, and clinical protocols. OBJECTIVES: Given the scarcity of studies assessing intraoral fluoride (F) retention after using such formulations, the present study evaluated the influence of pH and F concentration of dentifrices on F uptake by saliva, biofilm, and biofilm fluid. METHODS:Volunteers (n = 22) were randomly assigned to dentifrices containing 0 (placebo), 550 (LFD, low-fluoride dentifrice), and 1100 ppm F (CD, conventional dentifrice) at pH 4.5 and 7.0 and brushed their teeth 3 times/day following a double-blind, crossover protocol. Saliva and biofilm samples were collected after 7 days of using the dentifrices, 1 and approximately 12 h after last brushing. F and calcium (Ca) analyses were performed with the inverted electrode after buffering with TISAB III and the Arsenazo III method, respectively. Data were analyzed by 3-way ANOVA, Tukey's HSD test, and Pearson's correlation coefficient (p < 0.05). RESULTS:F concentrations in biofilm fluid and whole biofilm 1 h after brushing with acidic F-toothpastes were higher than those related to neutral counterparts, although the differences were small and not significant; no increases were observed in salivary F concentrations influenced by dentifrice pH. Moreover, no definite trend was observed for Ca concentrations in these compartments. CONCLUSION:Dentifrice pH had some influence on F uptake by the biofilm fluid, having lesser or no impact on F uptake by the biofilm and saliva, respectively. CLINICAL SIGNIFICANCE: Toothbrushing with acidic toothpastes leads to slight increases in F concentrations in the biofilm fluid when compared to neutral formulations, which may contribute to the higher anticaries effect of acidic formulations.
RCT Entities:
INTRODUCTION: Acidic dentifrices have been shown to be more effective than neutral ones against dental caries using in vitro, in situ, and clinical protocols. OBJECTIVES: Given the scarcity of studies assessing intraoral fluoride (F) retention after using such formulations, the present study evaluated the influence of pH and F concentration of dentifrices on F uptake by saliva, biofilm, and biofilm fluid. METHODS: Volunteers (n = 22) were randomly assigned to dentifrices containing 0 (placebo), 550 (LFD, low-fluoride dentifrice), and 1100 ppm F (CD, conventional dentifrice) at pH 4.5 and 7.0 and brushed their teeth 3 times/day following a double-blind, crossover protocol. Saliva and biofilm samples were collected after 7 days of using the dentifrices, 1 and approximately 12 h after last brushing. F and calcium (Ca) analyses were performed with the inverted electrode after buffering with TISAB III and the Arsenazo III method, respectively. Data were analyzed by 3-way ANOVA, Tukey's HSD test, and Pearson's correlation coefficient (p < 0.05). RESULTS:F concentrations in biofilm fluid and whole biofilm 1 h after brushing with acidic F-toothpastes were higher than those related to neutral counterparts, although the differences were small and not significant; no increases were observed in salivary F concentrations influenced by dentifrice pH. Moreover, no definite trend was observed for Ca concentrations in these compartments. CONCLUSION: Dentifrice pH had some influence on F uptake by the biofilm fluid, having lesser or no impact on F uptake by the biofilm and saliva, respectively. CLINICAL SIGNIFICANCE: Toothbrushing with acidic toothpastes leads to slight increases in F concentrations in the biofilm fluid when compared to neutral formulations, which may contribute to the higher anticaries effect of acidic formulations.
Authors: K M R P Alves; J P Pessan; F L Brighenti; K S Franco; F A L Oliveira; M A R Buzalaf; K T Sassaki; A C B Delbem Journal: Caries Res Date: 2007 Impact factor: 4.056
Authors: K P K Olympio; P A P Bardal; V E da S Cardoso; R C Oliveira; J R de M Bastos; M A R Buzalaf Journal: Caries Res Date: 2007 Impact factor: 4.056
Authors: R J Wierichs; H Zelck; C E Doerfer; P Appel; S Paris; M Esteves-Oliveira; H Meyer-Lueckel Journal: Odontology Date: 2016-02-05 Impact factor: 2.634