Merethe Hansen1, Michael T Lund1,2, Emilie Gregers1, Regitze Kraunsøe1, Gerrit Van Hall3,4, Jørn W Helge1, Flemming Dela1. 1. Xlab, Center for Healthy Aging, Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark. 2. Department of Surgery, Koege Hospital, Koege, Denmark. 3. Clinical Metabolomics Core Facility, Rigshospitalet, Copenhagen, Denmark. 4. Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark.
Abstract
OBJECTIVE: To study adipose tissue mitochondrial respiration and lipolysis following a massive weight loss. METHODS: High resolution respirometry of adipose tissue biopsies and tracer determined whole body lipolysis. Sixteen obese patients with type 2 diabetes (T2DM) and 27 without (OB) were studied following a massive weight loss by diet and Roux-en-Y gastric bypass (RYGB). RESULTS: The mitochondrial respiratory rates were similar in OB and T2DM, and the mass-specific oxygen flux increased significantly 4 and 18 months post-surgery (P < 0.05). With normalization to mitochondrial content, no differences in oxidative capacity after RYGB were seen. The ratio between the oxidative phosphorylation system capacity (P) and the capacity of the electron transfer system (E) increased 18 months after RYGB in both groups (P < 0.05). Lipolysis per fat mass was similar in the two groups and was increased (P < 0.05) and lipid oxidation during hyperinsulinemia decreased 4 months post-surgery. In T2DM, visceral fat mass was always higher relative to the body fat mass (%) compared to OB. CONCLUSIONS: Adipose tissue mitochondrial respiratory capacity increases with RYGB. Adipocytes adapt to massive weight loss by increasing the phosphorylation system ratio (P/E), suggesting an increased ability to oxidize substrates after RYGB. Lipolysis increases in the short term post-surgery, and insulin sensitivity for suppression of lipolysis increases with RYGB.
OBJECTIVE: To study adipose tissue mitochondrial respiration and lipolysis following a massive weight loss. METHODS: High resolution respirometry of adipose tissue biopsies and tracer determined whole body lipolysis. Sixteen obesepatients with type 2 diabetes (T2DM) and 27 without (OB) were studied following a massive weight loss by diet and Roux-en-Y gastric bypass (RYGB). RESULTS: The mitochondrial respiratory rates were similar in OB and T2DM, and the mass-specific oxygen flux increased significantly 4 and 18 months post-surgery (P < 0.05). With normalization to mitochondrial content, no differences in oxidative capacity after RYGB were seen. The ratio between the oxidative phosphorylation system capacity (P) and the capacity of the electron transfer system (E) increased 18 months after RYGB in both groups (P < 0.05). Lipolysis per fat mass was similar in the two groups and was increased (P < 0.05) and lipid oxidation during hyperinsulinemia decreased 4 months post-surgery. In T2DM, visceral fat mass was always higher relative to the body fat mass (%) compared to OB. CONCLUSIONS: Adipose tissue mitochondrial respiratory capacity increases with RYGB. Adipocytes adapt to massive weight loss by increasing the phosphorylation system ratio (P/E), suggesting an increased ability to oxidize substrates after RYGB. Lipolysis increases in the short term post-surgery, and insulin sensitivity for suppression of lipolysis increases with RYGB.
Authors: R Jokinen; R Rinnankoski-Tuikka; S Kaye; L Saarinen; S Heinonen; M Myöhänen; E Rappou; S Jukarainen; A Rissanen; A Pessia; V Velagapudi; K A Virtanen; E Pirinen; K H Pietiläinen Journal: Int J Obes (Lond) Date: 2017-12-05 Impact factor: 5.095
Authors: R G Vink; N J Roumans; M Čajlaković; J P M Cleutjens; M V Boekschoten; P Fazelzadeh; M A A Vogel; E E Blaak; E C Mariman; M A van Baak; G H Goossens Journal: Int J Obes (Lond) Date: 2017-02-09 Impact factor: 5.095
Authors: R Zamora-Mendoza; H Rosas-Vargas; M T Ramos-Cervantes; P Garcia-Zuniga; H Perez-Lorenzana; P Mendoza-Lorenzo; A C Perez-Ortiz; F J Estrada-Mena; A Miliar-Garcia; E Lara-Padilla; G Ceballos; A Rodriguez; F Villarreal; I Ramirez-Sanchez Journal: Int J Obes (Lond) Date: 2017-11-21 Impact factor: 5.095
Authors: Gregory N Ruegsegger; Ana L Creo; Tiffany M Cortes; Surendra Dasari; K Sreekumaran Nair Journal: J Clin Invest Date: 2018-08-31 Impact factor: 14.808
Authors: Henriette Frikke-Schmidt; Brian F Zamarron; Robert W O'Rourke; Darleen A Sandoval; Carey N Lumeng; Randy J Seeley Journal: Mol Metab Date: 2017-02-16 Impact factor: 7.422
Authors: Ioannis G Lempesis; Rens L J van Meijel; Konstantinos N Manolopoulos; Gijs H Goossens Journal: Acta Physiol (Oxf) Date: 2019-06-02 Impact factor: 6.311
Authors: Tiago Morais; Alexandre L Seabra; Bárbara G Patrício; Marta Guimarães; Mário Nora; Pedro F Oliveira; Marco G Alves; Mariana P Monteiro Journal: Int J Mol Sci Date: 2021-05-27 Impact factor: 5.923