A functional assessment of patients two years after lung transplantation in Poland.

THE AIM OF THE STUDY: The aim of the study was to assess the long-term results of lung transplantation (LT) in Poland two years after the procedure.
MATERIAL AND METHODS: The study included patients who underwent LT between December 2004 and December 2009 in the Silesian Center for Heart Diseases in Zabrze. Various lung functions (forced vital capacity - FVC; forced expiratory volume in 1 second - FEV1), the quality of life (SF-36 questionnaire), the level of perceived dyspnea (Medical Research Council - MRC; basic dyspnea index - BDI), and the patient's mobility (the 6-minute walking test - 6MWT) were assessed before and approximately 24 months after LT. Among 35 patients who underwent LT, 20 patients were referred to our study (mean age: 46.6 ± 9.03 years).
RESULTS: After LT, a statistically significant increase was observed in the distance achieved in the 6MWT (323.8 vs. 505.8 m), FVC (1.64 vs. 2.88 L), and FEV1 (1.37 vs. 2.09 L). An improvement in perceived dyspnea in MRC and BDI questionnaires was observed in patients with chronic obstructive pulmonary disease (COPD) after LT. The assessment of the quality of life, excluding perceived pain, showed the most significant improvement in the physical cumulative score (PCS; 25 vs. 45 points), especially in patients with idiopathic pulmonary fibrosis.
CONCLUSIONS: Lung transplantation in Poland, in patients who live longer than 2 years after the procedure, significantly improves the mobility, lung function, perceived dyspnea, and the quality of life.

Introduction

The first single lung transplantation was performed in the Silesian Center for Heart Diseases in Poland in April 2004 [1]. Since that time, lung transplantations (LTs) have been performed routinely in Poland, offering patients with end-stage pulmonary disease a viable option of survival. The Silesian Center for Heart Diseases has so far been the only medical center in Poland where lung transplantations are performed. Presently, more than 15 LTs are performed annually in Poland. Patients have been referred to the Department for Lung Diseases and Tuberculosis in Zabrze, Poland for LT since 1999. In the present study, we have analyzed the effectiveness of LT in all patients who were qualified for the procedure between the years 2004-2009 in the Department of Lung Diseases and Tuberculosis and who underwent the operation at the Silesian Center for Heart Diseases.

The aim of this study was to determine the results of LT two years after the operation. Hence, we assessed the respiratory function, mobility, quality of life, and level of dyspnea in all patients and compared the results with those from before the operation.

Material and methods

The study included all patients who underwent lung transplantation between December 2004 and December 2009 at the Silesian Center for Heart Diseases in Zabrze. During that time, 35 operations were performed (including 1 re-transplantation). Within this group, 12 patients (34%) died within 1 year after the operation and 2 patients (6%) remained in the hospital for over 6 months after the operation due to complications (bronchiectasis, aspergillosis). Ultimately, we included 20 patients in the study who fulfilled the assumed criteria (Table I).

Tab. I

Demographic data, date of referral for LT, date of LT, type of operation in each patient

Initials	Gender	Age	Diagnosis	Date of referral for LT	Date of LT	No. of waiting days from referral for LT	Type of operation
E.B.	M	54	IPF	10.12.2004	11.12.2004	1	SLT
G.J.	M	48	IPF	23.05.2005	17.03.2006	298	SLT
P.A.	M	31	Emphysema	02.11.2006	26.11.2006	24	DLT
K.K.	F	50	COPD	22.11.2006	06.12.2006	184	DLT
K.A.	M	49	IPF	09.03.2007	26.03.2007	16	SLT
G.A.	M	47	IPF	19.01.2007	23.07.2007	184	SLT
Ś.J.	M	61	IPF	03.07.2007	13.12.2007	163	SLT
T.A.	M	46	ILD	02.11.2006	14.12.2007	407	SLT
D.K.	M	36	Pneumoconiosis	19.17.2006	14.12.2007	513	SLT
Ż.E.	F	28	ILD	19.09.2006	05.06.2008	452	SLT
B.J.	M	50	IPF	08.01.2008	29.02.2008	52	SLT
J.A.	M	48	IPF	29.04.2008	15.11.2008	200	SLT
C.R.	M	48	ILD	22.07.2008	05.12.2008	136	DLT
B.M.	M	30	Emphysema	12.08.2008	06.12.2008	116	DLT
Z.K.	M	61	COPD	20.01.2009	16.03.2009	55	SLT
O.T.	F	53	COPD	23.10.2008	02.04.2009	161	SLT
Ś.M.	M	47	IPF	30.06.2008	09.06.2009	344	SLT
P.B.	M	50	IPF	06.08.2009	15.09.2009	40	SLT
M.A.	M	45	Bronchiectasis	28.02.2008	14.10.2009	116	SLT
W.A.	M	50	ILD	24.04.2007	06.04.2010	1137	SLT

LT – lung transplantation, SLT – single lung transplantation, DLT – double lung transplantation, IPF – idiopathic pulmonary fibrosis, ILD – interstitial lung disease other than IPF, COPD – chronic obstructive pulmonary disease

The criteria of qualification for the study included:

LT performed in the period 2004-2009,

time since LT = 24 months ± 2 months,

the possibility to perform study assessments during LT follow-up visits not related to the treatment of bronchiolitis obliterans syndrome (BOS) or other LT complications,

hospitalization was free of complications and no longer than 6 months in one year (the total time after LT).

The first examination was performed at the time of referral for LT. We assessed the following parameters: dyspnea, the quality of life, mobility, and respiratory function.

Assessment of dyspnea

The following clinical methods were used for dyspnea assessment:

the modified dyspnea scale of the United Kingdom Medical Research Council (MRC),

the Baseline Dyspnea Index (BDI) describing dyspnea in 5 steps integrated into 3 categories: a) functional impairment (FI); b) magnitude of task (MT); c) magnitude of effort (ME).

The details concerning the methodological aspects of dyspnea rating and quantification using the outlined methods have previously been described [2].

Assessment of quality of life

For this analysis, we used the SF-36 (Short-Form Health Survey), which belongs to a group of tools assessing the overall quality of life and is commonly used in many health service institutions.

The SF-36 questionnaire consists of 36 questions measuring the state of physical and mental health. It allows for the description of the physical quality of life via a Physical Cumulative Score (PCS), which consists of four domains: Physical Functioning (PF), Role Physical (RP) (limitations caused by physical health), Bodily Pain (BP), and General Health (GH). It also allows for the description of the mental quality of life via a Mental Cumulative Score (MCS), which consists of two domains: Role Emotional (RE) (limitations caused by mental health) and Mental Health (MH). The point scale is from 0 to 100 points; the higher the score in each domain, the higher the assessment of the quality of life. All study patients filled out the questionnaires on their own. The methodological rules and data analysis from the SF-36 questionnaire have been described previously [2].

Mobility

To estimate mobility, the 6-minute walking test (6MWT) was used. The test was performed according to the 2002 ATS guidelines [3]. Oxygen saturation was measured during the test. The test was performed twice on every patient within one day. For analysis, the best record from the two tests was used. The same rules were applied while performing the test after lung transplantation. The primary estimated parameter was the distance achieved during the test before and after the transplantation.

Respiratory function tests

The spirometric examination was performed using the Jaeger MasterLab (Erich-Jaeger GmbH, Wurzburg, Germany). The two parameters measured were forced vital capacity (FVC) and forced expiratory volume in 1 second (FEV1). The measurements were expressed as absolute values in L and as a percentage of predicted values. The values were normalized to reference values proposed by the European Community for Steel and Coal, and were subsequently introduced as a percentage of predicted values [4].

Statistical analysis

The statistical analysis included the calculation of mean, standard deviation, relevance level, and relevance of differences for each examined parameter, and was carried out using appropriate statistical software. Student's t-test was used for variables characterized by normal distribution and the nonparametric Wilcoxon test for paired samples for variables without normal distribution. The level of statistical significance was set at p < 0.05.

Results

Twenty patients who underwent LT between December 2004 and December 2009 were examined. The demographic data, the basic diagnosis which was the reason for LT evaluation, the type of LT executed, and the time spent waiting for a transplant for each patient are presented in Table I.

The examined group consisted of 17 men and 3 women with a mean age of 46.6 ± 9.03 years. The youngest patient was 28 years old and the oldest was 61 years old. The mean time of waiting for the transplant was 229.9 ± 260.5 days, and was between 1 to 1137 days. A single-lung transplantation was the most commonly performed procedure (16 SLtx, 4DLtx) (Table II).

Tab. II

Type of operation in relation to demographic data, waiting time, and diagnosis

Diagnosis Gender M/F	No. Age [years]	Age [years]	No. of waiting days for LT	Type of operation
Total	20 17 M/3 F	46.6 ± 9.03 min 28, max 61	229.9 ± 260.5 min 1, max 1137	20 4 DLT, 16 SLT
IPF	9 9 M	50.4 ± 4.5 min 47, max 61	144.2 ± 124.9 min 1, max 344	9 SLT
COPD	6 4 M/2 F	45.0 ± 12.4 min 30, max 61	109.3 ± 60.9 min 24, max 184	3 DLT, 3 SLT
ILD	5 4 M/1 F	41.6 ± 9.32 min 28, max 50	529.0 ± 369.2 min 136, max 1137	4 SLT, 1 DLT

LT – lung transplantation, SLT – single lung transplantation, DLT – double lung transplantation, IPF – idiopathic pulmonary fibrosis, ILD – interstitial lung disease other than IPF, COPD – chronic obstructive pulmonary disease

The lung transplantation was performed mainly on patients with idiopathic pulmonary fibrosis (IPF) (9 patients, 45%) and chronic obstructive pulmonary disease (COPD) (6 patients, 30%). The last group included patients with chronic interstitial pneumonia with etiology other than IPF (ILD, interstitial lung diseases) (5 patients, 25%). In this group, four patients had pulmonary fibrosis due to a chronic form of hypersensitivity pneumonitis and one patient developed pulmonary fibrosis and chronic respiratory failure in the course of silicosis. The longest waiting time for lung transplantation was observed in this group (1137 days). Patients with COPD had the shortest average waiting time of 109 ± 61 days. The shortest waiting time for transplantation was 1 day for a patient with IPF.

6-minute walking test

Before the transplantation, the average distance achieved during the 6-minute walking test (6MWT) was 323.8 ± 77.2 m (Table III), and all patients’ results were below normal values; the lowest observed value was not more than 10 m. After the transplantation, the 6MWT was not repeated for two patients. One of them (ZE) had complications including an ischemic stroke followed by paralysis of the limbs; the other patient had femoral artery embolus and, for this reason, needed a lower limb amputation. After the lung transplantation, a statistically significant increase (p < 0.0001) in distance was achieved, increasing from 323.8 ± 77.2 m to 505.8 ± 145.7 m. The results of the 6MWT for each patient before and after LT are shown in Figure 1. In 10 patients (50%), the distance achieved in the 6MWT after LT exceeded 500 m. A decrease in the distance achieved after LT was observed in two patients, while in the remaining group an increase in distance achieved was observed. The mean increase in the distance achieved was 181.9 ± 146.6 m.

Tab. III

Mean values of 6MWT, lung function tests, perceived dyspnea, and the quality of life in the study population before and after LT

	Patients referred for LT
	n	Before LT		After LT		Diff		p
		Mean	SD	Mean	SD	Mean	SD
6MWT	18	323.8	77.2	505.8	145.7	181.9	146.6	0.001
FVC	20	1.64	0.68	2.88	0.77	1.24	0.80	0.001
FVC%pred.	20	39.4	13.6	65.8	14.5	26.4	15.7	0.001
FEV1	20	1.37	0.81	2.09	0.59	0.72	0.81	0.003
FEV1%pred.	20	33.1	15.6	59.1	14.7	26.0	18.7	0.001
FEV1%VC	20	72.5	17.8	74.1	11.5	1.6	21.8	0.644
MRC	20	3.55	0.69	1.55	1.00	-2.00	1.08	0.001
FI/BDI	16	0.31	0.60	2.50	1.21	2.19	1.28	0.001
MT/BDI	19	0.74	0.65	2.84	1.12	2.11	1.37	0.001
ME/BDI	18	0.72	0.57	3.22	0.88	2.50	1.04	0.001
FI + MT + ME	20	1.70	1.53	7.90	2.83	6.20	3.16	0.001
PF	20	15.1	11.1	45.0	10.6	30.0	14.5	0.001
RP	20	20.5	24.7	42.1	11.7	21.6	29.1	0.005
BP	20	53.1	30.5	50.2	12.0	-2.9	29.5	0.911
GH	20	25.3	10.6	45.2	10.1	19.9	15.9	0.001
VT	20	39.8	15.1	55.9	11.7	16.1	18.6	0.001
SF	20	34.8	18.5	46.5	9.8	11.7	17.9	0.014
RE	20	23.6	24.8	44.4	15.8	20.8	26.2	0.003
MH	20	50.0	17.4	50.3	10.2	0.2	17.3	0.601
PCS	20	25.4	8.3	45.0	9.6	19.6	13.7	0.001
MCS	20	39.5	8.5	50.3	11.4	10.8	10.5	0.001

6MWT – 6 minute walking test, FVC – forced vital capacity, FEV1 – forced expiratory flow in 1 second, MRC – Medical Research Council, OCD – oxygen cost diagram, BDI – Baseline Dyspnea Index, FI – Functional Impairment, MT – Magnitude of Task, ME – Magnitude of Effort, PF – Physical Functioning, RP – Role Physical, BP – Bodily Pain, GH – General Health, VT – Vitality, SF – Social Functioning, RE – Role Emotional, MH – Mental Health, PCM – Physical Cumulative Score, MCS – Mental Cumulative Score

Fig. 1

Results of the 6MWT for each patient before and after LT

The largest increase in the distance achieved in the 6MWT was observed in patients with ILD (526 m), and the smallest in patients with IPF (82 m).

Lung function tests

Before LT, significant disorders of ventilatory efficiency were recorded in all study participants (FVC = 39.4 ± 13.6%pred., FEV1 = 33.1 ± 15.6%pred.). After the operations, the VC capacity observed had increased by twice as much as before the operations (1.64 ± 0.68 L vs. 2.88 ± 0.77 L, p < 0.05). The value of FEV1 increased from an average of 1.37 ± 0.81 L to 2.09 ± 0.59 L (p < 0.05). The largest increase in spirometric test values was noticed in patients with COPD (FVC%pred.: 36.2 vs. 74.09%).

Dyspnea analysis

Before the transplantation, the examined patients reported significant dyspnea in all estimated scales. In the MRC questionnaire before the transplantation, one patient reported maximum dyspnea (dyspnea appeared while dressing and during other daily activities) and 10 patients reported submaximal dyspnea (they were not able to walk a 90-m distance). After the LT, patients presented a significant improvement in the assessed dyspnea on the MRC scale (3.55 ± 0.69 vs. 1.55 ± 1.0, p = 0.001). The greatest improvement was in patients with COPD (2.33 points) and the lowest in those with IPF (1.78 points). In both of these groups, the improvement was statistically significant (p < 0.05).

A statistically significant improvement was also seen in the assessment of dyspnea by the BDI test. It was particularly visible in summary results of BDI (FI + MT + ME), where we reported a statistically significant (p = 0.001) improvement (1.70 ± 1.53 vs. 7.9 ± 2.83). Before the transplantation, each domain of the BDI test showed significant dyspnea in all patients, and their averages were not higher than 1.0. The largest deficiency before the transplantation was seen in the FI domain. The average in the examined group was 0.31 ± 0.60, which increased to 2.50 ± 1.21 (p = 0.001) after the transplantation. The largest increase was seen in patients with COPD, especially in the MT and ME domains (2.8 points and 3.25 points, respectively). The smallest increase was seen in the MT domain (1.6 point) in patients with ILD.

Quality of life

Before the transplantation, patients assessed their quality of life as low in all domains of the SF-36 questionnaire. The PCS before the transplantation was 25.4 ± 8.3 and the MCS was 39.5 ± 8.5 (Table III).

The biggest impairments the patients observed were in physical functioning (PF; 15.1 ± 11.1), limitations caused by physical health (RP; 20.5 ± 24.7), general health (GH; 25.3 ± 10.6), and limitations caused by mental health (RE; 23.6 ± 24.8) (Table III). After the lung transplantation, the only decrease in score observed in the domains assessing the quality of life was in bodily pain (BP, 53.1 ± 30.5 vs. 50.2 ± 12.0). Improvements were seen in all other domains of the SF-36 questionnaire. The biggest improvements were observed in the following domains: PF (15.1 ± 11.1 vs. 45.0 ± 10.6), RP (20.5 ± 24.7 vs. 42.1 ± 11.7), and RE (23.6 ± 24.8 vs. 44.4 ± 15.8). After lung transplantation, the total assessment of physical health (PCS) in the study group grew to 45.0 ± 9.6 and the total assessment of mental health (MCS) grew to 50.3 ± 11.4. By and large, the biggest increase in each domain of the SF-36 questionnaire was noted in the patient group with IPF (171 points). In this group, the biggest improvement was observed in PF (PF; + 31.9 points). The lowest overall improvement was observed in the patient group with ILD (98 points). The decrease in the scores for pain assessment (BP; –18.9) influenced this outcome.

Discussion

The present study presents the two-year outcome of lung transplantations performed at the Silesian Center for Heart Diseases in Zabrze in the years 2004-2009. This period covers the first years of the introduction of the Lung Transplantation Program in Poland. Patients who were qualified for LT in other medical centers were not included in the study, particularly the patients with primary lung hypertension and cystic fibrosis. The program for cystic fibrosis patients was introduced in 2010. In our study, 66% of the patients survived the period of two years after the transplantation. In comparison to Nathan's findings [5], we consider this a very good result, considering that the analyzed period includes the introduction of Poland's Lung Transplantation Program. The team of pulmonologists participating in qualifying patients for lung transplantation considers a very careful evaluation of patients an important factor helping to avoid the qualification of patients from a high-risk group for transplantation. Disqualifying patients in high-risk groups is a standard protocol whenever new therapeutic methods are introduced into the clinical practice. For example, the characteristics of high-risk patients for LT include: age over 65 years, chronic mechanical ventilation, and symptomatic osteoporosis [5]. In the presented material, the single lung transplantation dominated (SLTs, 80%). The Silesian Center for Heart Diseases in Poland started with SLTs, and is currently performing double lung transplantations (DLTs) as well.

The 6MWT is commonly accepted as an objective tool to estimate the physical effort ability in patients with cardiovascular diseases [3, 6]. The 6MWT distance achieved is an independent factor of survival chances for patients waiting for transplantation [7]. Tuppin et al. reported that patients who are not able to walk a 315-m distance have a higher risk of death, regardless of other factors such as age, weight, BMI, ejection fraction of the left ventricle of the heart, oxygen partial pressure in the blood, and the waiting time for transplantation [8]. In the studied group, the distance achieved in the 6MWT before the transplantation came to an average of 312.95 m. These were patients with reduced cardiovascular and general physical efficiency. After the transplantation, the average distance in the 6MWT test increased by almost 200 m, reaching the value of 505.8 m. Gerbase et al. assessed patients after LT over a 7-year observation period [9]. In their study, the average distance achieved in the 6MWT was 429 m one year after LT, and 433 m 3 years after. The increase in the distance achieved in the 6MWT was used by the authors as a basic parameter estimating the effectiveness of the performed operations, and is often assumed as a point of reference for other parameters such as physical activity and the quality of life [7-10]. The authors state that, even though one study patient displayed a statistically significant increase in the post-transplant achieved distance, it is rare that patients are able to cover a normal distance in the 6MWT after lung transplantation. This occurrence is explained by Bartels et al. [11], who reported the outcomes of the quality of life, respiratory function, and ergospirometric tests carried out on 153 patients over a 7-year observation period after lung transplantation. The authors showed that, despite an increase in ventilatory efficiency, the physical effort ability assessed by the ergospirometric tests in their study patients was significantly reduced and did not exceed 50 %pred. According to the authors, it is a consequence of distal muscle injury as a result of the supplied immunosuppression, muscle atrophy, and sustaining dyspnea rather than dysfunctions of the cardiovascular system. In our study, we observed similar circumstances. We observed a significant improvement of spirometric values and a significant improvement in the 6MWT results, although the patients’ reported dyspnea levels varied.

Lung transplantation is performed to prolong life and improve the quality of life in patients with irreversible lung injury in cases where pharmacological therapy does not bring improvement [5]. In the last years, a number of tools have been developed to estimate the quality of life in functional, psychological, and social aspects [12]. The questionnaire most often used to assess the quality of life is the SF-36 questionnaire. A comparative analysis of the outcomes of the SF-36 in our studied group with the assessments of the quality of life reported in the literature showed that patients similarly assessed Physical Functioning, Role Physical, General Health, and Vitality as worse before lung transplantation than after [13, 14]. This assessment was lower in the group of patients waiting for LT in comparison with patients waiting for kidney, liver, or bone marrow transplantations. It is believed that a low assessment of the quality of life in the Physical Functioning, Role Physical, General Health, and Vitality domains results from problems related to breathing and perceived dyspnea [15]. In these domains, the biggest improvement was observed. Similarly, in the assessed group of patients, an improvement of around 20 points was observed after transplantation in the domains of Role Physical, General Health, and Vitality after lung transplantation, and an improvement of 30 points was observed in the domain of Physical Functioning, which is not only statistically significant, but also defined as a clinical improvement in the SF-36 assessment manual [15]. Similar to other studies, the patients in our study group assessed their mental (psychical) health and pain ailments connected with the disease with the highest scores. In these domains, we observed, as did other authors [13, 14], the smallest improvement after the transplantation. In the domain of Bodily Pain, patients reported a deterioration after the transplantation. This aspect is important, as it was carried out two years after the transplantation and it should not be connected with the surgery. It is supposed that the experienced pain is connected with immunosuppression, which has many side effects, such as osteoporosis. Assessing the general perception of the quality of life in the estimated group after the transplantation, we conclude that, in comparison with the data presented by other authors, the quality of life is lower [9, 10, 13, 14]. The value did not exceed 55 points in any of the domains of the SF-36 questionnaire taken after the transplantation, while in such domains as Social Functioning and Emotional State, other authors observed values over 80 points. Perhaps patients in Poland are exposed to greater stress and suffering, which results in a lower assessment of their quality of life. The study showed that the majority of patients had a survival rate of more than 2 years and an improvement of the general and ventilatory ability. The obtained data do not differ from the results presented in other centers. Based on these positive results, we find that Poland's current number of lung transplantations, 15 per year in a population of 37 million residents, is insufficient. We conclude that it is necessary to develop and expand the lung transplantation program in Poland.