Sung-Suk Suh1, Mirye Park1,2, Jinik Hwang1,2, Eui-Joon Kil3, Sukchan Lee3, Taek-Kyun Lee4,5. 1. South Sea Environment Research Department, Korea Institute of Ocean Science & Technology, Geoje, 656-830, Korea. 2. Marine Environmental Chemistry and Biology, Korea University of Science and Technology, Daejeon, 305-350, Korea. 3. Department of Genetic Engineering, Sungkyunkwan University, Suwon, 440-746, Republic of Korea. 4. South Sea Environment Research Department, Korea Institute of Ocean Science & Technology, Geoje, 656-830, Korea. tklee@kiost.ac. 5. Marine Environmental Chemistry and Biology, Korea University of Science and Technology, Daejeon, 305-350, Korea. tklee@kiost.ac.
Abstract
OBJECTIVES: To detect Cochlodinum polykrikoides in long-term monitoring and high-throughput sampling projects using an integrated sandwich hybridization and nuclease protection assay (NPA-SH). RESULTS: The specificity of the probes was verified with individual and mixed cultures as well as field collection, and the quantity of C. polykrikoides determined by NPA-SH analysis showed a good correlation with that determined by cell-counting with a light microscope. In addition a standard curve for C. polykrikoides was established to represent the correlation between optical absorbance in the NPA-SH assay and cell density. CONCLUSIONS: This approach provides an efficient alternative to traditional, morphology-based methods for the rapid identification and quantification of harmful algal species and could be used to monitor phytoplankton in field surveys.
OBJECTIVES: To detect Cochlodinum polykrikoides in long-term monitoring and high-throughput sampling projects using an integrated sandwich hybridization and nuclease protection assay (NPA-SH). RESULTS: The specificity of the probes was verified with individual and mixed cultures as well as field collection, and the quantity of C. polykrikoides determined by NPA-SH analysis showed a good correlation with that determined by cell-counting with a light microscope. In addition a standard curve for C. polykrikoides was established to represent the correlation between optical absorbance in the NPA-SH assay and cell density. CONCLUSIONS: This approach provides an efficient alternative to traditional, morphology-based methods for the rapid identification and quantification of harmful algal species and could be used to monitor phytoplankton in field surveys.