| Literature DB >> 26334866 |
Weihua Song1, Chee Wai Fhu1, Koon Hwee Ang2, Cheng Hao Liu1, Nurul Azizah Binte Johari1, Daniel Lio1, Sabu Abraham3, Wanjin Hong2, Stephen E Moss4, John Greenwood4, Xiaomeng Wang1,2,4.
Abstract
The mouse fetal metatarsal provides a unique tool for studying angiogenesis. In comparison with other commonly used in vitro or ex vivo angiogenesis assays, vessel outgrowth from mouse fetal metatarsals is more representative of sprouting angiogensis in vivo. It allows the analysis of blood vessel growth, and the mechanisms underpinning this process, in a multicellular microenvironment that drives the formation of a robust and complex vascular network in the absence of exogenous growth factors. By labeling different constituents of the vascular structure, it is possible to perform 3D rendering of the spatial interplay between different cellular components and to carry out quantitative analysis of vessel outgrowth. High-resolution imaging permits the visualization of fine structural and cellular details. As the assay involves the use of fetal tissues, it is possible to follow new blood vessel formation in genetically modified mice that are perinatally lethal. The entire process takes 9-13 d. A detailed description of how to set up and perform the assay is described here.Entities:
Mesh：
Year: 2015 PMID： 26334866 DOI： 10.1038/nprot.2015.097
Source DB: PubMed Journal: Nat Protoc ISSN： 1750-2799 Impact factor: 13.491