H Neymeyer1, R Labes1, V Reverte2, F Saez2, T Stroh3, C Dathe1, S Hohberger1, M Zeisberg4, G A Müller4, J Salazar2, S Bachmann1, A Paliege1,5. 1. Department of Anatomy, Charité Universitätsmedizin Berlin, Berlin, Germany. 2. Department of Physiology, School of Medicine, University of Murcia, Murcia, Spain. 3. Department of Medicine, Charité Universitätsmedizin Berlin, Berlin, Germany. 4. Department of Nephrology and Rheumatology, Göttingen University Medical Center, Göttingen, Germany. 5. Department of Nephrology, Charité Universitätsmedizin Berlin, Berlin, Germany.
Abstract
AIM: The anti-inflammatory protein annexin A1 (AnxA1) and its formyl peptide receptor 2 (FPR2) have protective effects in organ fibrosis. Their role in chronic kidney disease (CKD) has not yet been elucidated. Our aim was to characterize the AnxA1/FPR2 system in models of renal fibrosis. METHODS: Rats were treated with angiotensin receptor antagonist during the nephrogenic period (ARAnp) to induce late-onset hypertensive nephropathy and fibrosis. Localization and regulation of AnxA1 and FPR2 were studied by quantitative real-time PCR and double labelling immunofluorescence. Biological effects of AnxA1 were studied in cultured renal fibroblasts from AnxA1(-/-) and wild-type mice. RESULTS: Angiotensin receptor antagonist during the nephrogenic period kidneys displayed matrix foci containing CD73(+) fibroblasts, alpha-smooth muscle actin (a-SMA)(+) myofibroblasts and CD68(+) macrophages. TGF-β and AnxA1 mRNAs were ~threefold higher than in controls. AnxA1 was localized to macrophages and fibroblasts; myofibroblasts were negative. FPR2 was localized to fibroblasts, myofibroblasts, macrophages and endothelial cells. AnxA1 and FPR2 immunoreactive signals were increased in the foci, with fibroblasts and macrophages expressing both proteins. AnxA1(-/-) fibroblasts revealed higher α-SMA (sevenfold) and collagen 1A1 (Col1A1; 144-fold) mRNA levels than controls. Treatment of murine WT fibroblasts with TGF-β (22.5 ng mL 24 h(-1)) increased mRNA levels of α-SMA (9.3-fold) and Col1A1 (fourfold). These increases were greatly attenuated upon overexpression of AnxA1 (1.5- and 1.7-fold, respectively; P < 0.05). Human fibroblasts reacted similarly when receiving the FPR2 inhibitor WRW4. CONCLUSION: Our results demonstrate that AnxA1 and FPR2 are abundantly expressed in the renal interstitium and modulate fibroblast phenotype and extracellular matrix synthesis activity.
AIM: The anti-inflammatory protein annexin A1 (AnxA1) and its formyl peptide receptor 2 (FPR2) have protective effects in organ fibrosis. Their role in chronic kidney disease (CKD) has not yet been elucidated. Our aim was to characterize the AnxA1/FPR2 system in models of renal fibrosis. METHODS:Rats were treated with angiotensin receptor antagonist during the nephrogenic period (ARAnp) to induce late-onset hypertensive nephropathy and fibrosis. Localization and regulation of AnxA1 and FPR2 were studied by quantitative real-time PCR and double labelling immunofluorescence. Biological effects of AnxA1 were studied in cultured renal fibroblasts from AnxA1(-/-) and wild-type mice. RESULTS: Angiotensin receptor antagonist during the nephrogenic period kidneys displayed matrix foci containing CD73(+) fibroblasts, alpha-smooth muscle actin (a-SMA)(+) myofibroblasts and CD68(+) macrophages. TGF-β and AnxA1 mRNAs were ~threefold higher than in controls. AnxA1 was localized to macrophages and fibroblasts; myofibroblasts were negative. FPR2 was localized to fibroblasts, myofibroblasts, macrophages and endothelial cells. AnxA1 and FPR2 immunoreactive signals were increased in the foci, with fibroblasts and macrophages expressing both proteins. AnxA1(-/-) fibroblasts revealed higher α-SMA (sevenfold) and collagen 1A1 (Col1A1; 144-fold) mRNA levels than controls. Treatment of murine WT fibroblasts with TGF-β (22.5 ng mL 24 h(-1)) increased mRNA levels of α-SMA (9.3-fold) and Col1A1 (fourfold). These increases were greatly attenuated upon overexpression of AnxA1 (1.5- and 1.7-fold, respectively; P < 0.05). Human fibroblasts reacted similarly when receiving the FPR2 inhibitor WRW4. CONCLUSION: Our results demonstrate that AnxA1 and FPR2 are abundantly expressed in the renal interstitium and modulate fibroblast phenotype and extracellular matrix synthesis activity.
Authors: Fadi W Adel; Aruna Rikhi; Siu-Hin Wan; Seethalakshmi R Iyer; Hrishikesh Chakraborty; Steven McNulty; W H Wilson Tang; G Michael Felker; Michael M Givertz; Horng H Chen Journal: J Card Fail Date: 2020-05-27 Impact factor: 5.712
Authors: Helena Ribeiro Souza; Lucas Ribeiro de Azevedo; Lucas Possebon; Sara de Souza Costa; Melina Mizusaki Iyomasa-Pilon; Sonia Maria Oliani; Ana Paula Girol Journal: PLoS One Date: 2017-03-09 Impact factor: 3.240
Authors: Francisco J Rios; Zhi-Guo Zou; Adam P Harvey; Katie Y Harvey; Ryszard Nosalski; Panagiota Anyfanti; Livia L Camargo; Silvia Lacchini; Alexey G Ryazanov; Lillia Ryazanova; Sarah McGrath; Tomasz J Guzik; Carl S Goodyear; Augusto C Montezano; Rhian M Touyz Journal: Cardiovasc Res Date: 2020-03-01 Impact factor: 10.787
Authors: Arseniy E Yuzhalin; Su Yin Lim; Alex N Gordon-Weeks; Roman Fischer; Benedikt M Kessler; Dihua Yu; Ruth J Muschel Journal: Am J Physiol Gastrointest Liver Physiol Date: 2019-09-23 Impact factor: 4.052