Alistair Robson1, Zena Shukur1, Mina Ally2, Justine Kluk1, Kun Liu3, Laura Pincus4, Debjani Sahni5, Uma Sundram6, Antonio Subtil7, Laszlo Karai8, Werner Kempf9, Stefan Schieke10, Philip Coates11. 1. St John's Institute of Dermatology, London, UK. 2. Department of Dermatology, Stanford University, Stanford, CA, USA. 3. Division of Health and Social Care Research, Kings College London, London, UK. 4. Department of Pathology, UCSF, San Francisco, CA, USA. 5. Department of Dermatology, Boston University Medical Center, Boston, MA, USA. 6. Department of Pathology, Stanford University, Stanford, CA, USA. 7. Dermatopathology Department, Yale University School of Medicine, New Haven, CT, USA. 8. Lake Diagnostics Florida, Lake City, FL, USA. 9. Department of Dermatology, University Hospital Zurich, Zurich, Switzerland. 10. Department of Dermatology, Medical College of Wisconsin, Milwaukee, WI, USA. 11. Dundee Cancer Centre, Ninewells Hospital and Medical School, Dundee, UK.
Abstract
AIMS: The p63 gene shares structural and functional homologies with the p53 family of transcriptional activators, but differs in exhibiting a consistent expression pattern in normal tissues. Although p63 is rarely mutated in malignancy studies of primary human tumours and cell lines suggest that p63 may promote tumour development. In non-Hodgkin's nodal lymphoma, TAp63 expression in follicular lymphoma (54%) and diffuse large B cell lymphoma (34%) has been described and correlated with the proliferative index. In this study, we analysed a series of primary cutaneous B cell lymphomas for immunohistochemical expression of p63. METHODS AND RESULTS: Thirty cases of diffuse large B cell lymphoma leg type (pcDLBCLL) and 34 cases of follicle centre cell lymphoma (pcFCCL) were stained using a generic antibody to p63, and a subset of these with an antibody specific for delta-Np63 isoform. The results indicate a significant difference between pcDLBCLL (21 of 30) and pcFCCL (four of 34) in p63 expression (P = 0.000); expression correlated strongly with the proliferation rate as assessed by Ki-67 (P = 0.015). None of the p63((+)) cases tested expressed the delta-Np63 isoform, suggesting that expression is of the TAp63 isoform. CONCLUSIONS: Functional studies are required to clarify the significance of p63 overexpression in primary cutaneous B cell lymphoma.
AIMS: The p63 gene shares structural and functional homologies with the p53 family of transcriptional activators, but differs in exhibiting a consistent expression pattern in normal tissues. Although p63 is rarely mutated in malignancy studies of primary humantumours and cell lines suggest that p63 may promote tumour development. In non-Hodgkin's nodal lymphoma, TAp63 expression in follicular lymphoma (54%) and diffuse large B cell lymphoma (34%) has been described and correlated with the proliferative index. In this study, we analysed a series of primary cutaneous B cell lymphomas for immunohistochemical expression of p63. METHODS AND RESULTS: Thirty cases of diffuse large B cell lymphoma leg type (pcDLBCLL) and 34 cases of follicle centre cell lymphoma (pcFCCL) were stained using a generic antibody to p63, and a subset of these with an antibody specific for delta-Np63 isoform. The results indicate a significant difference between pcDLBCLL (21 of 30) and pcFCCL (four of 34) in p63 expression (P = 0.000); expression correlated strongly with the proliferation rate as assessed by Ki-67 (P = 0.015). None of the p63((+)) cases tested expressed the delta-Np63 isoform, suggesting that expression is of the TAp63 isoform. CONCLUSIONS: Functional studies are required to clarify the significance of p63 overexpression in primary cutaneous B cell lymphoma.
Authors: Xueju Wang; Rebecca L Boddicker; Surendra Dasari; Jagmohan S Sidhu; Marshall E Kadin; William R Macon; Stephen M Ansell; Rhett P Ketterling; Karen L Rech; Andrew L Feldman Journal: Hum Pathol Date: 2017-01-30 Impact factor: 3.466
Authors: Stefan Steurer; Claudia Riemann; Franziska Büscheck; Andreas M Luebke; Martina Kluth; Claudia Hube-Magg; Andrea Hinsch; Doris Höflmayer; Sören Weidemann; Christoph Fraune; Katharina Möller; Anne Menz; Margit Fisch; Michael Rink; Christian Bernreuther; Patrick Lebok; Till S Clauditz; Guido Sauter; Ria Uhlig; Waldemar Wilczak; David Dum; Ronald Simon; Sarah Minner; Eike Burandt; Rainer Krech; Till Krech; Andreas H Marx Journal: Biomark Res Date: 2021-01-25