| Literature DB >> 26330014 |
Junyu He1, Guangchao Zhu1, Lu Gao2, Pan Chen1, Yuehua Long2, Shan Liao2, Hong Yi3, Wei Yi2, Zhen Pei2, Minghua Wu1, Xiaoling Li1, Juanjuan Xiang1, Shuping Peng1, Jian Ma1, Ming Zhou1, Wei Xiong1, Zhaoyang Zeng1, Bo Xiang1, Ke Tang2, Li Cao2, Guiyuan Li1, Yanhong Zhou1.
Abstract
Gastric cancer is an aggressive disease that continues to have a daunting impact on global health. Fra-1 (FOSL1) plays important roles in oncogenesis in various malignancies. We investigated the expression of Fra-1 in gastric cancer (GC) tissues by qPCR, immunohistochemistry (IHC) and western blot technologies. The results showed that Fra-1 was overexpressed in gastric cancer tissues compared with the adjacent non‑cancerous tissues. To explore the possible mechanism of Fra-1 in GC, we elucidated the effect of Fra-1 in the apoptosis and cell cycle of gastric cancer cells, AGS, and found that a considerable decrease in apoptotic cells and increase of S phase rate were observed for AGS cells with Fra-1 overexpession. We identified and confirmed that Fra-1 affected the expression level of CTTN and EZR in vitro through LC-MS/MS analyses and western blot technology. Furthermore, we found that Fra-1 was correlated with dysregulation PI3K/Akt and p53 signaling pathway in gastric cancer tissues in vitro. Moreover, we found that Fra-1 overexpression affected the expression of PI3K, Akt, MDM2 and p53 in vivo. In summary, our results suggest that Fra-1 is upregulated in gastric cancer tissues and plays its function by affecting the PI3K/Akt and p53 signaling pathway in gastric cancer.Entities:
Mesh：
Substances：
Year: 2015 PMID： 26330014 DOI： 10.3892/ijo.2015.3146
Source DB: PubMed Journal: Int J Oncol ISSN： 1019-6439 Impact factor: 5.650