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Literature DB >> 26324944

Subunit composition of a DEG/ENaC mechanosensory channel of Caenorhabditis elegans.

Yushu Chen¹, Shashank Bharill², Ehud Y Isacoff², Martin Chalfie³.

Abstract

Caenorhabditis elegans senses gentle touch in the six touch receptor neurons (TRNs) using a mechanotransduction complex that contains the pore-forming degenerin/epithelial sodium channel (DEG/ENaC) proteins MEC-4 and MEC-10. Past work has suggested these proteins interact with the paraoxonase-like MEC-6 and the cholesterol-binding stomatin-like MEC-2 proteins. Using single molecule optical imaging in Xenopus oocytes, we found that MEC-4 forms homotrimers and MEC-4 and MEC-10 form 4:4:10 heterotrimers. MEC-6 and MEC-2 do not associate tightly with these trimers and do not influence trimer stoichiometry, indicating that they are not part of the core channel transduction complex. Consistent with the in vitro data, MEC-10, but not MEC-6, formed puncta in TRN neurites that colocalize with MEC-4 when MEC-4 is overexpressed in the TRNs.

Entities: Chemical Gene Species

Keywords: DEG/ENaC proteins; channel stoichiometry; mechanosensory channels

Mesh：

Substances：

Year: 2015 PMID： 26324944 PMCID： PMC4577206 DOI： 10.1073/pnas.1515968112

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

40 in total

1. Podocin and MEC-2 bind cholesterol to regulate the activity of associated ion channels.

Authors: Tobias B Huber; Bernhard Schermer; Roman Ulrich Müller; Martin Höhne; Malte Bartram; Andrea Calixto; Henning Hagmann; Christian Reinhardt; Fabienne Koos; Karl Kunzelmann; Elena Shirokova; Dietmar Krautwurst; Christian Harteneck; Matias Simons; Hermann Pavenstädt; Dontscho Kerjaschki; Christoph Thiele; Gerd Walz; Martin Chalfie; Thomas Benzing
Journal: Proc Natl Acad Sci U S A Date: 2006-11-01 Impact factor: 11.205

Review 2. Neurosensory mechanotransduction.

Authors: Martin Chalfie
Journal: Nat Rev Mol Cell Biol Date: 2009-01 Impact factor: 94.444

3. Rules of engagement for NMDA receptor subunits.

Authors: Maximilian H Ulbrich; Ehud Y Isacoff
Journal: Proc Natl Acad Sci U S A Date: 2008-09-08 Impact factor: 11.205

4. The DEG/ENaC protein MEC-10 regulates the transduction channel complex in Caenorhabditis elegans touch receptor neurons.

Authors: Jóhanna Arnadóttir; Robert O'Hagan; Yushu Chen; Miriam B Goodman; Martin Chalfie
Journal: J Neurosci Date: 2011-08-31 Impact factor: 6.167

10. Pore-lining residues of MEC-4 and MEC-10 channel subunits tune the Caenorhabditis elegans degenerin channel's response to shear stress.

Authors: Shujie Shi; Stephanie M Mutchler; Brandon M Blobner; Ossama B Kashlan; Thomas R Kleyman
Journal: J Biol Chem Date: 2018-05-09 Impact factor: 5.157

Subunit composition of a DEG/ENaC mechanosensory channel of Caenorhabditis elegans.

1. Podocin and MEC-2 bind cholesterol to regulate the activity of associated ion channels.

Review 2. Neurosensory mechanotransduction.

3. Rules of engagement for NMDA receptor subunits.

4. The DEG/ENaC protein MEC-10 regulates the transduction channel complex in Caenorhabditis elegans touch receptor neurons.

5. The evolution of function in strictosidine synthase-like proteins.

6. Functional architecture of olfactory ionotropic glutamate receptors.

7. Pickpocket is a DEG/ENaC protein required for mechanical nociception in Drosophila larvae.

8. Spatial asymmetry in the mechanosensory phenotypes of the C. elegans DEG/ENaC gene mec-10.

9. Gain-of-function mutations in the MEC-4 DEG/ENaC sensory mechanotransduction channel alter gating and drug blockade.

10. Probing cellular protein complexes using single-molecule pull-down.

1. The Drosophila Postsynaptic DEG/ENaC Channel ppk29 Contributes to Excitatory Neurotransmission.

2. Investigation of mechanosensation in C. elegans using light field calcium imaging.

3. The RTK Interactome: Overview and Perspective on RTK Heterointeractions.

Review 4. Discoveries in structure and physiology of mechanically activated ion channels.

5. Functional features of the "finger" domain of the DEG/ENaC channels MEC-4 and UNC-8.

6. Composition and Control of a Deg/ENaC Channel during Presynaptic Homeostatic Plasticity.

7. Ca2+ permeability and Na+ conductance in cellular toxicity caused by hyperactive DEG/ENaC channels.

8. Activation of the Caenorhabditis elegans Degenerin Channel by Shear Stress Requires the MEC-10 Subunit.

9. Regulation of the epithelial Na⁺ channel by paraoxonase-2.

10. Pore-lining residues of MEC-4 and MEC-10 channel subunits tune the Caenorhabditis elegans degenerin channel's response to shear stress.