Literature DB >> 26324437

Immuno-Electron Microscopy and Electron Microscopic In Situ Hybridization for Visualizing piRNA Biogenesis Bodies in Drosophila Ovaries.

Shinsuke Shibata1, Yukiko Murota, Yoshinori Nishimoto, Mana Yoshimura, Toshihiro Nagai, Hideyuki Okano, Mikiko C Siomi.   

Abstract

Immuno-electron microscopy and electron microscopic in situ hybridization are powerful tools to identify the precise subcellular localization of specific proteins and RNAs at the ultramicroscopic level. Here we describe detailed procedures for how to detect the precise location of a specific target labeled with both fluorescence and gold particles. Although they have been developed for the analysis of Drosophila ovarian somatic cells, these techniques are suitable for a wide range of biological applications including human, primate, and rodent analysis.

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Year:  2015        PMID： 26324437     DOI： 10.1007/978-1-4939-2851-4_12

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

1.  Region- and Cell Type-Specific Facilitation of Synaptic Function at Destination Synapses Induced by Oligodendrocyte Depolarization.

Authors:  Yoshihiko Yamazaki; Yoshifumi Abe; Shinsuke Shibata; Tomoko Shindo; Satoshi Fujii; Kazuhiro Ikenaka; Kenji F Tanaka
Journal:  J Neurosci       Date:  2019-03-12       Impact factor: 6.167

2.  Obesity-induced kidney injury is attenuated by amelioration of aberrant PHD2 activation in proximal tubules.

Authors:  Koji Futatsugi; Hirobumi Tokuyama; Shinsuke Shibata; Makiko Naitoh; Takeshi Kanda; Hitoshi Minakuchi; Shintaro Yamaguchi; Koichi Hayashi; Yoji Andrew Minamishima; Motoko Yanagita; Shu Wakino; Hiroshi Itoh
Journal:  Sci Rep       Date:  2016-11-09       Impact factor: 4.379

3.  Pathological processes in aqueous humor due to iris atrophy predispose to early corneal graft failure in humans and mice.

Authors:  Takefumi Yamaguchi; Kazunari Higa; Yukari Yagi-Yaguchi; Koji Ueda; Hisashi Noma; Shinsuke Shibata; Toshihiro Nagai; Daisuke Tomida; Ririko Yasu-Mimura; Osama Ibrahim; Ryo Matoba; Kazuo Tsubota; Pedram Hamrah; Jun Yamada; Kohsuke Kanekura; Jun Shimazaki
Journal:  Sci Adv       Date:  2020-05-13       Impact factor: 14.136

4.  Nav1.2 haplodeficiency in excitatory neurons causes absence-like seizures in mice.

Authors:  Ikuo Ogiwara; Hiroyuki Miyamoto; Tetsuya Tatsukawa; Tetsushi Yamagata; Tojo Nakayama; Nafiseh Atapour; Eriko Miura; Emi Mazaki; Sara J Ernst; Dezhi Cao; Hideyuki Ohtani; Shigeyoshi Itohara; Yuchio Yanagawa; Mauricio Montal; Michisuke Yuzaki; Yushi Inoue; Takao K Hensch; Jeffrey L Noebels; Kazuhiro Yamakawa
Journal:  Commun Biol       Date:  2018-07-19

5.  Large-Area Fluorescence and Electron Microscopic Correlative Imaging With Multibeam Scanning Electron Microscopy.

Authors:  Shinsuke Shibata; Taro Iseda; Takayuki Mitsuhashi; Atsushi Oka; Tomoko Shindo; Nobuko Moritoki; Toshihiro Nagai; Shinya Otsubo; Takashi Inoue; Erika Sasaki; Chihiro Akazawa; Takao Takahashi; Richard Schalek; Jeff W Lichtman; Hideyuki Okano
Journal:  Front Neural Circuits       Date:  2019-05-08       Impact factor: 3.492

6.  Isoform-specific mutation in Dystonin-b gene causes late-onset protein aggregate myopathy and cardiomyopathy.

Authors:  Nozomu Yoshioka; Masayuki Kurose; Masato Yano; Dang Minh Tran; Shujiro Okuda; Yukiko Mori-Ochiai; Masao Horie; Toshihiro Nagai; Ichizo Nishino; Shinsuke Shibata; Hirohide Takebayashi
Journal:  Elife       Date:  2022-08-09       Impact factor: 8.713

7.  Application of q-Space Diffusion MRI for the Visualization of White Matter.

Authors:  Kanehiro Fujiyoshi; Keigo Hikishima; Jin Nakahara; Osahiko Tsuji; Junichi Hata; Tsunehiko Konomi; Toshihiro Nagai; Shinsuke Shibata; Shinjiro Kaneko; Akio Iwanami; Suketaka Momoshima; Shinichi Takahashi; Masahiro Jinzaki; Norihiro Suzuki; Yoshiaki Toyama; Masaya Nakamura; Hideyuki Okano
Journal:  J Neurosci       Date:  2016-03-02       Impact factor: 6.167

8.  Human Spinal Oligodendrogenic Neural Progenitor Cells Promote Functional Recovery After Spinal Cord Injury by Axonal Remyelination and Tissue Sparing.

Authors:  Narihito Nagoshi; Mohamad Khazaei; Jan-Eric Ahlfors; Christopher S Ahuja; Satoshi Nori; Jian Wang; Shinsuke Shibata; Michael G Fehlings
Journal:  Stem Cells Transl Med       Date:  2018-08-07       Impact factor: 6.940

9.  Stem cells purified from human induced pluripotent stem cell-derived neural crest-like cells promote peripheral nerve regeneration.

Authors:  Hiroo Kimura; Takehito Ouchi; Shinsuke Shibata; Tsuyoshi Amemiya; Narihito Nagoshi; Taneaki Nakagawa; Morio Matsumoto; Hideyuki Okano; Masaya Nakamura; Kazuki Sato
Journal:  Sci Rep       Date:  2018-07-03       Impact factor: 4.379
  9 in total

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