| Literature DB >> 26322960 |
Tao Hou1,2, Chi Wang1,2, Zhili Ma1,2, Wen Shi1,2, Lui Weiwei1,2, Hui He1,2.
Abstract
The effects of desalted duck egg white peptides (DPs) on calcium absorption were investigated in three models: Caco-2 cell monolayer model, Caco-2 cell population model, and everted intestinal sac model. DPs were found to enhance calcium transport and may do so by acting as calcium carriers and interacting with the cell membrane to open a special Ca(2+) channel, whereas the paracellular pathway may make only a minor contribution. Structure characterization demonstrated the important roles of seven crucial peptides, such as VSEE and LYAEE, in binding calcium and promoting calcium uptake. Three synthetic peptides (VHSS, VSEE, and VHS(p)S(p)) potently induced calcium transport in Caco-2 monolayers, with VHS(p)S(p) being the most effective. This research expands the understanding of the mechanism of cellular calcium uptake by DPs as well as highlights an opportunity for recycling an otherwise discarded processing byproduct.Entities:
Keywords: Caco-2 cells; calcium uptake; desalted duck egg white peptides (DPs); everted intestinal sac model; structure characterization
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Year: 2015 PMID： 26322960 DOI： 10.1021/acs.jafc.5b03097
Source DB: PubMed Journal: J Agric Food Chem ISSN： 0021-8561 Impact factor: 5.279