Xiaoying Wang1, Lili Chen2, Ting Wang2, Xiaoqing Jiang2, Han Zhang2, Pan Li2, Bin Lv2, Xiumei Gao3. 1. College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China; State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China. 2. State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China. 3. State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China. Electronic address: gaoxiumei@tjutcm.edu.cn.
Abstract
BACKGROUND: Adriamycin (ADM) is an antineoplastic agent that is effective against a wide range of cancers, but cardiac toxicity limits its clinical application. Ginsenoside Rg3 (Rg3), an anti-cancer active ingredient of Panax ginseng, was reported to have anti-oxidative, anti-apoptotic, and cardioprotective properties. PURPOSE: The current study aimed to investigate the possible protective effect of Rg3 against ADM-induced cardiotoxicity. STUDY DESIGN: The activity of Rg3 to improve endothelial dysfunction was processed both in vivo and in vitro. METHODS: We investigated the cardioprotective effect of Rg3 on ADM treated rats by echocardiography. The endothelial dysfunction was assessed using an aortic ring assay. Cardiac microvascular endothelial cells were cultured to investigate the effects of Rg3 on ADM-treated cells. RESULTS: Results showed that Rg3 could ameliorate the decrease in the ejection fraction and fractional shortening that was induced by ADM, and improve the left ventricular outflow. The aortic ring assay showed that Rg3 could partially recover the abnormal vascular function. In vitro studies showed that Rg3 could promote cell viability to attenuate ADM induced oxidative damage and apoptosis. This counteraction was achieved partially via activation of the Nrf2-ARE pathway through the activation of Akt. CONCLUSION: These findings elucidated the potential of Rg3 as a promising reagent for treating ADM-induced cardiotoxicity in clinic.
BACKGROUND:Adriamycin (ADM) is an antineoplastic agent that is effective against a wide range of cancers, but cardiac toxicity limits its clinical application. Ginsenoside Rg3 (Rg3), an anti-cancer active ingredient of Panax ginseng, was reported to have anti-oxidative, anti-apoptotic, and cardioprotective properties. PURPOSE: The current study aimed to investigate the possible protective effect of Rg3 against ADM-induced cardiotoxicity. STUDY DESIGN: The activity of Rg3 to improve endothelial dysfunction was processed both in vivo and in vitro. METHODS: We investigated the cardioprotective effect of Rg3 on ADM treated rats by echocardiography. The endothelial dysfunction was assessed using an aortic ring assay. Cardiac microvascular endothelial cells were cultured to investigate the effects of Rg3 on ADM-treated cells. RESULTS: Results showed that Rg3 could ameliorate the decrease in the ejection fraction and fractional shortening that was induced by ADM, and improve the left ventricular outflow. The aortic ring assay showed that Rg3 could partially recover the abnormal vascular function. In vitro studies showed that Rg3 could promote cell viability to attenuate ADM induced oxidative damage and apoptosis. This counteraction was achieved partially via activation of the Nrf2-ARE pathway through the activation of Akt. CONCLUSION: These findings elucidated the potential of Rg3 as a promising reagent for treating ADM-induced cardiotoxicity in clinic.
Authors: Lan Li; Jingyu Ni; Min Li; Jingrui Chen; Lifeng Han; Yan Zhu; Deling Kong; Jingyuan Mao; Yi Wang; Boli Zhang; Meifeng Zhu; Xiumei Gao; Guanwei Fan Journal: Drug Deliv Date: 2017-11 Impact factor: 6.419
Authors: Mengqi Guo; Jie Xiao; Xi Sheng; Xinyu Zhang; Yuanyuan Tie; Lei Wang; Lang Zhao; Xiaoping Ji Journal: Front Pharmacol Date: 2018-05-09 Impact factor: 5.810