Monia Deghrigue1, Carmen Festa2, Lotfi Ghribi3, Maria Valeria D'Auria2, Simona De Marino2, Hichem Ben Jannet3, Abderrahman Bouraoui4. 1. Laboratoire de développement chimique, galénique et pharmacologique des médicaments (LR12ES09). Equipe de Pharmacologie marine, Faculté de pharmacie de Monastir, Université de Monastir, 5000 Monastir, Tunisia. Electronic address: moniadeghrigue@yahoo.fr. 2. Department of Pharmacy, University of Naples "Federico II", via D. Montesano 49, I- 80131 Napoli, Italy. 3. Laboratoire de chimie hétérocyclique, produits naturels et réactivité. Equipe de chimie médicinale et produits naturels (LR11ES39), Faculté des sciences de Monastir, Université de Monastir, 5000 Monastir, Tunisia. 4. Laboratoire de développement chimique, galénique et pharmacologique des médicaments (LR12ES09). Equipe de Pharmacologie marine, Faculté de pharmacie de Monastir, Université de Monastir, 5000 Monastir, Tunisia.
Abstract
OBJECTIVE: To explore anti-inflammatory activities of organic extract and its semi-purified fractions (ethanol, acetone, methanol/dichloromethane) from the Mediterranean gorgonian Eunicella singularis. METHODS: The anti-inflammatory and analgesic activities were evaluated, using the carrageenan-induced rat paw edema model and the acetic acid writhing test in mice. The gastroprotective activity was determined using HCl/EtOH induced gastric ulcers in rats. The purification and structure elucidation of compound(s) from the more effective fraction were determined by chromatographic and spectroscopic methods and in comparison with data reported in the literature. RESULTS: The fraction F-EtOH showed an important anti-inflammatory activity associated with significant analgesic and gastroprotective properties. The purification and structure elucidation of compound(s) from this fraction lead to the identification of one diterpenoid and four sterols. CONCLUSIONS: These results suggested that components from the active fraction can be used to treat various anti-inflammatory diseases.
OBJECTIVE: To explore anti-inflammatory activities of organic extract and its semi-purified fractions (ethanol, acetone, methanol/dichloromethane) from the Mediterranean gorgonian Eunicella singularis. METHODS: The anti-inflammatory and analgesic activities were evaluated, using the carrageenan-induced rat paw edema model and the acetic acid writhing test in mice. The gastroprotective activity was determined using HCl/EtOH induced gastric ulcers in rats. The purification and structure elucidation of compound(s) from the more effective fraction were determined by chromatographic and spectroscopic methods and in comparison with data reported in the literature. RESULTS: The fraction F-EtOH showed an important anti-inflammatory activity associated with significant analgesic and gastroprotective properties. The purification and structure elucidation of compound(s) from this fraction lead to the identification of one diterpenoid and four sterols. CONCLUSIONS: These results suggested that components from the active fraction can be used to treat various anti-inflammatory diseases.
Authors: Sophia A Kolesnikova; Ekaterina G Lyakhova; Anatoly I Kalinovsky; Roman S Popov; Ekaterina A Yurchenko; Valentin A Stonik Journal: Mar Drugs Date: 2018-11-21 Impact factor: 5.118