| Literature DB >> 26321301 |
Janine Ebner1, Anson V Koehler2, Gemma Robertson3, Richard S Bradbury4, Aaron R Jex2, Shane R Haydon5, Melita A Stevens5, Robert Norton3, Anja Joachim6, Robin B Gasser7.
Abstract
To date, there has been limited genetic study of the gastrointestinal pathogens Giardia and Cryptosporidium in northern parts of Australia. Here, PCR-based methods were used for the genetic characterization of Giardia and Cryptosporidium from 695 people with histories of gastrointestinal disorders from the tropical North of Australia. Genomic DNAs from fecal samples were subjected to PCR-based analyses of regions from the triose phosphate isomerase (tpi), small subunit (SSU) of the nuclear ribosomal RNA and/or the glycoprotein (gp60) genes. Giardia and Cryptosporidium were detected in 13 and four of the 695 samples, respectively. Giardia duodenalis assemblages A and B were found in 4 (31%) and 9 (69%) of the 13 samples in persons of <9 years of age. Cryptosporidium hominis (subgenotype IdA18), Cryptosporidium mink genotype (subgenotype IIA16R1) and C. felis were also identified in single patients of 11-21 years of age. Future studies might focus on a comparative study of these and other protists in rural communities in Northern Australia.Entities:
Keywords: Cryptosporidium; Giardia; Northern Australia; PCR-based analyses; Pathogen; Sequencing
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Year: 2015 PMID： 26321301 DOI： 10.1016/j.meegid.2015.08.034
Source DB: PubMed Journal: Infect Genet Evol ISSN： 1567-1348 Impact factor: 3.342