| Literature DB >> 26321292 |
Yutaka Konya1, Takeshi Bamba1, Eiichiro Fukusaki2.
Abstract
An extra-facile chiral liquid chromatography-time of flight mass spectrometry (LC-TOFMS) analytical method of amino acid enantiomers has been developed without a derivatization process. The enantioseparation of eighteen proteinogenic amino acids (except for proline) was simultaneously performed using a combination of a chiral column (CROWNPAK CR-I(+)) and a TOFMS within 15 min. An isocratic condition of a simple mobile phase comprising acetonitrile/water/trifluoroacetic acid (96/4/0.5) gave baseline separation of all underivatized amino acid enantiomers on the chiral column.Entities:
Keywords: Chiral liquid chromatography; Time of flight mass spectrometry; Underivatized amino acids; d-Amino acids
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Year: 2015 PMID： 26321292 DOI： 10.1016/j.jbiosc.2015.06.017
Source DB: PubMed Journal: J Biosci Bioeng ISSN： 1347-4421 Impact factor: 2.894