Wei Peng1, Xiao-Qian Qiu2, Zhi-Heng Shu3, Qing-Chun Liu4, Mei-Bian Hu4, Ting Han3, Khalid Rahman5, Lu-Ping Qin6, Cheng-Jian Zheng7. 1. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China; College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, PR China. 2. Department of Stomatology, Changhai Hospital, Second Military Medical University, Shanghai 200433, PR China. 3. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China. 4. College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, PR China. 5. Faculty of Science, School of Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool, L3 3 AF England, UK. 6. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China. Electronic address: qinsmmu@126.com. 7. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China. Electronic address: zhengchengjian@smmu.edu.cn.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Currently, human liver is susceptible to injury caused by alcohol and virus infiltration, resulting in hepatitis, cirrhosis, and even hepatocellular carcinoma. Paederia scandens (Lour.) Merr. var. tomentosa (Rubiaceae) has been used as traditional medicine in Asian countries to treat jaundice, dysentery, and abdominal mass. Furthermore, the abundance of iridoid glycosides in Paederia species indicates their notable hepatoprotective potential. MATERIALS AND METHODS: Total iridoid glycosides (TG) was prepared, and constituents of TG were analyzed by HPLC. TG and silymarin (positive) were orally administered for 15 days. Then, acute liver injury rats was induced by intraperitoneally injection (i.p.) of 10% CCl4 (0.12%, v/v, dissolved in olive oil, 10 mL/kg, body weight). Rats were sacrificed at 16 h after CCl4 injection. Liver tissues and blood were collected. Serum samples were prepared to determine the activities of alanine transaminase (ALT) and aspartate transaminase (AST), whereas liver tissue sections were prepared for the purpose of examining possible liver histopathological changes. In addition, antioxidant enzyme activities in liver tissues were also evaluated. RESULTS: Our results demonstrated that TG significantly decreased the levels of AST and ALT, compared with those in control rats. In addition, pre-treatment of the rats with TG clearly alleviated their liver tissue injuries. What's more, the activities of GSH, GAT and SOD in the groups of TG-treated rats were significantly increased compared with those of rats in the control group, whereas the levels of MDA were decreased. CONCLUSIONS: Our present research indicated that TG possessed notable hepatoprotective activity via decreasing oxidative stress level in liver tissues.
ETHNOPHARMACOLOGICAL RELEVANCE: Currently, human liver is susceptible to injury caused by alcohol and virus infiltration, resulting in hepatitis, cirrhosis, and even hepatocellular carcinoma. Paederia scandens (Lour.) Merr. var. tomentosa (Rubiaceae) has been used as traditional medicine in Asian countries to treat jaundice, dysentery, and abdominal mass. Furthermore, the abundance of iridoid glycosides in Paederia species indicates their notable hepatoprotective potential. MATERIALS AND METHODS:Total iridoid glycosides (TG) was prepared, and constituents of TG were analyzed by HPLC. TG and silymarin (positive) were orally administered for 15 days. Then, acute liver injuryrats was induced by intraperitoneally injection (i.p.) of 10% CCl4 (0.12%, v/v, dissolved in olive oil, 10 mL/kg, body weight). Rats were sacrificed at 16 h after CCl4 injection. Liver tissues and blood were collected. Serum samples were prepared to determine the activities of alanine transaminase (ALT) and aspartate transaminase (AST), whereas liver tissue sections were prepared for the purpose of examining possible liver histopathological changes. In addition, antioxidant enzyme activities in liver tissues were also evaluated. RESULTS: Our results demonstrated that TG significantly decreased the levels of AST and ALT, compared with those in control rats. In addition, pre-treatment of the rats with TG clearly alleviated their liver tissue injuries. What's more, the activities of GSH, GAT and SOD in the groups of TG-treated rats were significantly increased compared with those of rats in the control group, whereas the levels of MDA were decreased. CONCLUSIONS: Our present research indicated that TG possessed notable hepatoprotective activity via decreasing oxidative stress level in liver tissues.
Authors: Adriano Costa de Camargo; Alina Concepción Alvarez; María Fernanda Arias-Santé; Juan Esteban Oyarzún; Marcelo E Andia; Sergio Uribe; Paula Núñez Pizarro; Simón M Bustos; Andrés R Schwember; Fereidoon Shahidi; Raquel Bridi Journal: Antioxidants (Basel) Date: 2022-06-10