| Literature DB >> 26319599 |
Yan Gu1, Xuan Zhang2, Qian Yang1, Jian-mei Wang3, Ya-ping He4, Zhao-gui Sun4, Hui-qin Zhang1, Jian Wang5.
Abstract
Entities:
Year: 2015 PMID: 26319599 PMCID: PMC4552999 DOI: 10.1186/s12958-015-0089-x
Source DB: PubMed Journal: Reprod Biol Endocrinol ISSN: 1477-7827 Impact factor: 5.211
Fig. 5In vitro decidualization of mouse primary ESCs. ESCs isolated from the uteri of day 4 pregnant mice were cultured in the presence of P4 and E2. a Immunocytochemical detection of vimentin and cytokeratin in cultured ESCs. b Quantitative PCR analysis of DTPRP mRNA expression in ESCs cultured for up to 72 h. c Western blot analysis of NDRG2 protein expression in ESCs cultured for up to 72 h. Densitometric analyses of NDRG2 at each time point compared with 0 h is shown. d Quantitative PCR analysis of NDRG2 mRNA expression in cultured ESCs. The relative fold induction of NDRG2 mRNA expression at each time point compared with its expression in the 0 h sample is shown. The values represent the mean ± SEM, as determined from three separate experiments. *, significantly different (P < 0.05)