RuiPing Gu1, Min Zhou1, ChunHui Jiang1,2, Jian Yu1, GeZhi Xu1. 1. Department of Ophthalmology and Shanghai Key Laboratory of Visual Impairment and Restoration, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China. 2. Department of Ophthalmology, No. 5 People's Hospital of Shanghai, Shanghai, China.
Abstract
BACKGROUND: We aim to study the level of inflammatory cytokines in aqueous of post-vitrectomy eyes. METHODS: Aqueous samples were obtained from post-vitrectomy eyes that underwent cataract surgery. Samples from age-related cataract patients were also collected as controls. The concentrations of cytokines were measured by array-based multiplex sandwich enzyme-linked immunosorbent assay system and microparticle-based multiplex cytokine assay. RESULTS: Eighteen post-vitrectomy eyes from 18 patients were included; all received one vitrectomy with gas injection for rhegmatogenous retinal detachment. The mean interval between vitrectomy and cataract surgery was 22.33 months (range 6 to 80). Primary results show that in post-vitrectomy eyes the levels of many cytokines, such as B-lymphocyte chemo-attractant, I-309, interleukin (IL)-6, IL-8, interferon γ-induced protein-10 and intercellular adhesion molecule 1, were higher than control. Further analysis demonstrated that the levels of IL-8, interferon γ-induced protein-10 and intercellular adhesion molecule 1 were significantly elevated in post-vitrectomy eyes, even after the subjects were divided by their refractive state. CONCLUSIONS: The concentrations of some cytokines were increased in the anterior chambers of post-vitrectomy eyes even a long time after vitreous surgery. This surge in post-operative cytokines might cause changing in the micro-environment of the anterior, and this might contribute to the development of secondary glaucoma or fasten the development of cataract in these eyes.
BACKGROUND: We aim to study the level of inflammatory cytokines in aqueous of post-vitrectomy eyes. METHODS: Aqueous samples were obtained from post-vitrectomy eyes that underwent cataract surgery. Samples from age-related cataractpatients were also collected as controls. The concentrations of cytokines were measured by array-based multiplex sandwich enzyme-linked immunosorbent assay system and microparticle-based multiplex cytokine assay. RESULTS: Eighteen post-vitrectomy eyes from 18 patients were included; all received one vitrectomy with gas injection for rhegmatogenous retinal detachment. The mean interval between vitrectomy and cataract surgery was 22.33 months (range 6 to 80). Primary results show that in post-vitrectomy eyes the levels of many cytokines, such as B-lymphocyte chemo-attractant, I-309, interleukin (IL)-6, IL-8, interferon γ-induced protein-10 and intercellular adhesion molecule 1, were higher than control. Further analysis demonstrated that the levels of IL-8, interferon γ-induced protein-10 and intercellular adhesion molecule 1 were significantly elevated in post-vitrectomy eyes, even after the subjects were divided by their refractive state. CONCLUSIONS: The concentrations of some cytokines were increased in the anterior chambers of post-vitrectomy eyes even a long time after vitreous surgery. This surge in post-operative cytokines might cause changing in the micro-environment of the anterior, and this might contribute to the development of secondary glaucoma or fasten the development of cataract in these eyes.