Dan-Yu Wang1, Wei-Ming Li2, Meng-Di Jing1, Hai-Yan Cui1, Bo Lu1, Xin Wang1, Ze-Lin Liu3. 1. Department of Hematology, Nanshan Hospital Affiliated to Guangdong Medical College, Shenzhen 518052, Guangdong Province, China. 2. Department of Hematology, Affiliated Union Hospital of Tongji Medical College, Huazhong University of Scienes and Techonolgy, Wuhan 430022, Hubei Province, China. 3. Department of Hematology, Nanshan Hospital Affiliated to Guangdong Medical College, Shenzhen 518052, Guangdong Province, China. E-mail: liuzelin1962@163.com.
Abstract
UNLABELLED: Objetive: To investigate the effects of PKF118-310 on cell cycle and proliferation of K562 cell lines and its mechanism. METHODS: After treatment of PKF118-310 with different concentration, the proliferation inhibition on K562 cell lines was detected by MTT, the existance of β-catenin and TCF-4 in the cells was observed by immunohistochemistry. The change of the cell cycle was detected by flow cytometry. The expressions of caspase-3, β-catenin, TCF and BCL-9 were detected by Western blot. RESULTS: PKF118-310 can inhibit the proliferation of K562 cell line by S phase blocking. The β-catenin and TCF in the cells were observed by immunohistochemistry. After treating this cell line with PKF118-310 of different concentrations for 72 h, the expression level of caspase-3 increased, the expression levels of β-catenin, TCF and BCL-9 significantly decreased. CONCLUSION: PKF118-310 induces cycle arest of K562 cells at the S phase and inhibits the proliferation of these cells through decreasing β-catenin/TCF/BCL-9 thrascriptional activity.
UNLABELLED: Objetive: To investigate the effects of PKF118-310 on cell cycle and proliferation of K562 cell lines and its mechanism. METHODS: After treatment of PKF118-310 with different concentration, the proliferation inhibition on K562 cell lines was detected by MTT, the existance of β-catenin and TCF-4 in the cells was observed by immunohistochemistry. The change of the cell cycle was detected by flow cytometry. The expressions of caspase-3, β-catenin, TCF and BCL-9 were detected by Western blot. RESULTS: PKF118-310 can inhibit the proliferation of K562 cell line by S phase blocking. The β-catenin and TCF in the cells were observed by immunohistochemistry. After treating this cell line with PKF118-310 of different concentrations for 72 h, the expression level of caspase-3 increased, the expression levels of β-catenin, TCF and BCL-9 significantly decreased. CONCLUSION: PKF118-310 induces cycle arest of K562 cells at the S phase and inhibits the proliferation of these cells through decreasing β-catenin/TCF/BCL-9 thrascriptional activity.