Jian Zhu1, Qing Ye2, Li Chang2, Wei Xiong2, Qingqing He1, Wenhui Li2. 1. Department of Thyroid and Breast Surgery, General Hospital of Jinan Military Command Jinan, China. 2. Department of Radiation Oncology, The Third Affiliated Hospital of Kunming Medical University, Tumor Hospital of Yunnan Province Kunming, China.
Abstract
AIMS: To investigate the effect of miR-195 overexpression on radiosensitivity and the relevant molecular mechanisms in breast cancer. METHODS: miR-195 mimics were transfected to breast cancer cell line MCF-7 by gene transfection; miR-195 expression level in cells was determined by real-time quantitative PCR. Cellular viability was observed by colony formation assay after the cells were irradiated with X-ray. Apoptosis was detected by annexin V/propidium iodide (PI) staining. Protein expression was evaluated by Western blot assay. RESULTS: After miR-195 mimics were transfected, miR-195 expression in breast cancer cells was significantly upregulated (P < 0.05). miR-195 overexpression significantly enhanced the response to radiation of MCF-7 cells with a decrease in colony survival at individual doses of X-ray (P < 0.05). After the cells were irradiated with 5 Gy, apoptotic rate was significantly increased in miR-195-overexpressed group with a rate of 18.13% ± 1.57%; moreover, BCL-2 protein expression was significantly downregulated (P < 0.05). CONCLUSION: Exogenetic miR-195 expression could significantly enhance the radiosensitivity of human breast cancer cells; this finding may be attributed to BCL-2 downregulation to promote radiation-induced apoptosis.
AIMS: To investigate the effect of miR-195 overexpression on radiosensitivity and the relevant molecular mechanisms in breast cancer. METHODS:miR-195 mimics were transfected to breast cancer cell line MCF-7 by gene transfection; miR-195 expression level in cells was determined by real-time quantitative PCR. Cellular viability was observed by colony formation assay after the cells were irradiated with X-ray. Apoptosis was detected by annexin V/propidium iodide (PI) staining. Protein expression was evaluated by Western blot assay. RESULTS: After miR-195 mimics were transfected, miR-195 expression in breast cancer cells was significantly upregulated (P < 0.05). miR-195 overexpression significantly enhanced the response to radiation of MCF-7 cells with a decrease in colony survival at individual doses of X-ray (P < 0.05). After the cells were irradiated with 5 Gy, apoptotic rate was significantly increased in miR-195-overexpressed group with a rate of 18.13% ± 1.57%; moreover, BCL-2 protein expression was significantly downregulated (P < 0.05). CONCLUSION: Exogenetic miR-195 expression could significantly enhance the radiosensitivity of humanbreast cancer cells; this finding may be attributed to BCL-2 downregulation to promote radiation-induced apoptosis.
Entities:
Keywords:
Breast cancer; apoptosis; miR-195; radiosensitivity
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