Sae-Rom Yoo1, Chang-Seob Seo1, Ohn-Soon Kim2, Hyeun-Kyoo Shin1, Soo-Jin Jeong2. 1. K-herb Research Center, Korea Institute of Oriental Medicine Daejeon, Republic of Korea. 2. KM Convergence Research Division, Korea Institute of Oriental Medicine Daejeon, Republic of Korea.
Abstract
AIMS: Here we report in vitro anti-adipogenic and antioxidant effects of Samchulgeonbi-tang (SCGBT), a traditional Korean herbal formula. METHODS: 3T3-L1 preadipocytes were differentiated into adipocytes with or without SCGBT. After differentiation, we measured Oil Red O staining, glycerol-3-phosphate dehydrogenase (GPDH) activity and leptin production. In addition, its effect on scavenging activities of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radicals in in vitro systems. RESULTS: In differentiated 3T3-L1 adipocytes, SCGBT significantly inhibited lipid accumulation and triglyceride production, and mediated inactivation of GPDH, a major enzyme in the process of adipogenesis. Consistent with this, SCGBT stimulation significantly decreased the amount of leptin in 3T3-L1 adipose cells. Furthermore, SCGBT enhanced the scavenging activities on ABTS and DPPH radicals. The generation of malondialdehyde (MDA) during low-density lipoprotein (LDL) oxidation was significantly reduced by SCGBT treatment. Of interest, SCGBT extract inhibited reactive oxygen species (ROS) generation in 3T3-L1 adipocytes. CONCLUSION: Overall, our findings suggest that SCGBT has the potential for anti-adipogenic activity and antioxidant properties.
AIMS: Here we report in vitro anti-adipogenic and antioxidant effects of Samchulgeonbi-tang (SCGBT), a traditional Korean herbal formula. METHODS: 3T3-L1 preadipocytes were differentiated into adipocytes with or without SCGBT. After differentiation, we measured Oil Red O staining, glycerol-3-phosphate dehydrogenase (GPDH) activity and leptin production. In addition, its effect on scavenging activities of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radicals in in vitro systems. RESULTS: In differentiated 3T3-L1 adipocytes, SCGBT significantly inhibited lipid accumulation and triglyceride production, and mediated inactivation of GPDH, a major enzyme in the process of adipogenesis. Consistent with this, SCGBT stimulation significantly decreased the amount of leptin in 3T3-L1 adipose cells. Furthermore, SCGBT enhanced the scavenging activities on ABTS and DPPH radicals. The generation of malondialdehyde (MDA) during low-density lipoprotein (LDL) oxidation was significantly reduced by SCGBT treatment. Of interest, SCGBT extract inhibited reactive oxygen species (ROS) generation in 3T3-L1 adipocytes. CONCLUSION: Overall, our findings suggest that SCGBT has the potential for anti-adipogenic activity and antioxidant properties.
Entities:
Keywords:
Anti-adipogenic; Samchulgeonbi-tang; antioxidant; korean herbal formula