Regulatory T cells and IL-17(+) T helper cells enhanced in patients with chronic hepatitis B virus infection.

Hepatitis B is a worldwide infectious disease caused by hepatitis B virus (HBV), it leaded to millions of deaths every year, HBV mainly through immune response to damage liver cells. The purpose of this study was to judge the value of Regulatory T cells (Treg) and IL-17(+) T helper cells (Th17) in different chronic HBV infection stages. 96 patients with chronic HBV infection were enrolled and selected 33 healthy adults as control. Detected the expression of Treg and Th17 cells in peripheral blood by flow cytometry and assayed liver function simultaneously. Compared to the control group, the expression of Treg (6.80±1.92 vs. 4.42±0.97; P<0.0001) and Th17 (6.15±4.20 vs. 2.66±1.79; P<0.0001) cells were both increased and the ratio of Treg/Th17 was significantly decreased (1.48±0.89 vs. 2.29±1.31; P=0.0001) in patients with HBV infection. Spearman correlation analysis demonstrated that the level of Treg and Th17 cells were associated with liver function. ROC curve analysis found that Treg and Th17 cells were suitable for as a screening test for early detection of the disease. In conclusion, the expression of Treg and Th17 cells were increased in chronic hepatitis B patients and these indicators were independent risk factors to hepatitis.