Sebnem Ozemri Sag1, Tahsin Yakut1, Ozlem Gorukmez2, Orhan Gorukmez2, Mehmet Ture1, Mutlu Karkucak3, Tuna Gulten1, Ridvan Ali4. 1. 1 Department of Medical Genetics, Faculty of Medicine, Uludag University , Bursa, Turkey . 2. 2 Department of Medical Genetics, Sevket Yılmaz Education and Research Hospital , Bursa, Turkey . 3. 3 Department of Medical Genetics, Sakarya Education and Research Hospital , Sakarya, Turkey . 4. 4 Division of Hematology, Department of Internal Medicine, Faculty of Medicine, Uludag University , Bursa, Turkey .
Abstract
AIMS: Fluorescence in situ hybridization (FISH) and quantitative real-time polymerase chain reaction (QRT-PCR) were used to diagnose or screen for minimal residual disease (MRD) in Philadelphia (Ph) chromosome-positive leukemia. We compared the diagnostic utility of FISH and QRT-PCR at various time points in the course of chronic myelogenous leukemia (CML) and to determine the mean initial values for patients whose QRT-PCR results were not known at the time of diagnosis. RESULTS: We analyzed 135 results for 78 CML patients tested by FISH and QRT-PCR for the Ph chromosomal translocation. All newly diagnosed cases were positive by both methods. On follow-up following treatment, 1 case was FISH positive and QRT-PCR negative; 61 cases were FISH negative and QRT-PCR positive. Overall concordance was 54.1%. There was good concordance between QRT-PCR results and cytogenetic response categories. CONCLUSIONS: We confirmed that QRT-PCR allows precise measurement of low levels of BCR-ABL transcripts and can serve as a sensitive indicator of MRD. We also demonstrated 100% correlation between QRT-PCR and FISH in newly diagnosed CML.
AIMS: Fluorescence in situ hybridization (FISH) and quantitative real-time polymerase chain reaction (QRT-PCR) were used to diagnose or screen for minimal residual disease (MRD) in Philadelphia (Ph) chromosome-positive leukemia. We compared the diagnostic utility of FISH and QRT-PCR at various time points in the course of chronic myelogenous leukemia (CML) and to determine the mean initial values for patients whose QRT-PCR results were not known at the time of diagnosis. RESULTS: We analyzed 135 results for 78 CMLpatients tested by FISH and QRT-PCR for the Ph chromosomal translocation. All newly diagnosed cases were positive by both methods. On follow-up following treatment, 1 case was FISH positive and QRT-PCR negative; 61 cases were FISH negative and QRT-PCR positive. Overall concordance was 54.1%. There was good concordance between QRT-PCR results and cytogenetic response categories. CONCLUSIONS: We confirmed that QRT-PCR allows precise measurement of low levels of BCR-ABL transcripts and can serve as a sensitive indicator of MRD. We also demonstrated 100% correlation between QRT-PCR and FISH in newly diagnosed CML.
Authors: Wafaa M Rashed; Mohamed A Kandeil; Mohamed O Mahmoud; Doha Maher; Sameera Ezzat; Mohamed H Abdel-Rahman Journal: J Cancer Res Clin Oncol Date: 2020-09-26 Impact factor: 4.553