High-efficiency transfection of suspension cell lines.

Transfection of suspension cells has proven to be very difficult using conventional methods. Here, we present a simple and time-saving new transfection protocol wherein cell culture plates coated with chicken egg white are seeded with suspension cells prior to transfection. Our results demonstrate that coupling egg white coatings with commercially available transfection reagents leads to high transfection efficiency with suspension cell lines including canine mastocytoma C2 and the human myeloid cell line HL-60. This new approach, which should prove applicable to a wide range of cell lines, solves a crucial problem for researchers working with suspension cells.