Ivana Musilova1, Lubomira Tothova2,3, Ramkumar Menon4, Barbora Vlkova2,3, Peter Celec2,3, Helena Hornychova5, Radka Kutova6, Ctirad Andrys7, Martin Stepan1, Marian Kacerovsky1,8. 1. a Department of Obstetrics and Gynecology , Charles University in Prague, Faculty of Medicine Hradec Kralove , Hradec Kralove , Czech Republic . 2. b Institute of Molecular Biomedicine, Comenius University in Bratislava , Bratislava , Slovak Republic . 3. c Center for Molecular Medicine, Slovak Academy of Sciences , Bratislava , Slovak Republic . 4. d Department of Obstetrics and Gynecology , The University of Texas Medical Branch at Galveston , Galveston , TX , USA . 5. e Fingerland'S Department of Pathology, Charles University in Prague, Faculty of Medicine Hradec Kralove , University Hospital Hradec Kralove , Hradec Kralove , Czech Republic . 6. f Department of Clinical Biochemistry , Charles University in Prague, Faculty of Medicine Hradec Kralove, University Hospital Hradec Kralove , Hradec Kralove , Czech Republic . 7. g Department of Clinical Immunology and Allergy , Charles University in Prague, Faculty of Medicine Hradec Kralove, University Hospital Hradec Kralove , Hradec Kralove , Czech Republic , and. 8. h Biomedical Research Center, University Hospital Hradec Kralove , Hradec Kralove , Czech Republic.
Abstract
OBJECTIVE: To determine umbilical cord blood total antioxidant capacity (TAC), ferric reducing antioxidant power (FRAP), thiobarbituric acid-reacting substances (TBARS), advanced glycation end products (AGEs) and markers of oxidative stress in pregnancies complicated by preterm prelabor rupture of membranes (PPROM) and their associations with microbial invasion of the amniotic cavity (MIAC) and/or histological chorioamnionitis (HCA), funisitis and selected aspects of short-term neonatal morbidity. MATERIALS AND METHODS: One hundred and sixty-five women with singleton pregnancies complicated by PPROM were included in this study. Blood samples were obtained by venipuncture from the umbilical cord vein after the delivery of the newborn. The umbilical cord blood concentrations of TAC, FRAP, TBARS and AGEs were measured. RESULTS: The presence of MIAC, HCA and funisitis did not show differences in the umbilical cord blood TAC, FRAP, TBARS and AGEs concentrations. Positive correlations were found between the gestational age at sampling and umbilical cord blood TAC and AGEs concentrations (TAC: rho = 0.26; p = 0.001; AGEs: rho = 0.35; p < 0.0001). There was no association between umbilical cord blood TAC, FRAP, TBARS and AGEs concentrations and selected aspects of short-term neonatal morbidity. CONCLUSIONS: Oxidative stress is associated with PPROM, as indicated by the presence of markers tested in the umbilical cord blood; however, the evaluated oxidative stress markers are not influenced by the presence of MIAC and/or HCA, and funisitis or subsequent development of selected aspects of short-term neonatal morbidity.
OBJECTIVE: To determine umbilical cord blood total antioxidant capacity (TAC), ferric reducing antioxidant power (FRAP), thiobarbituric acid-reacting substances (TBARS), advanced glycation end products (AGEs) and markers of oxidative stress in pregnancies complicated by preterm prelabor rupture of membranes (PPROM) and their associations with microbial invasion of the amniotic cavity (MIAC) and/or histological chorioamnionitis (HCA), funisitis and selected aspects of short-term neonatal morbidity. MATERIALS AND METHODS: One hundred and sixty-five women with singleton pregnancies complicated by PPROM were included in this study. Blood samples were obtained by venipuncture from the umbilical cord vein after the delivery of the newborn. The umbilical cord blood concentrations of TAC, FRAP, TBARS and AGEs were measured. RESULTS: The presence of MIAC, HCA and funisitis did not show differences in the umbilical cord blood TAC, FRAP, TBARS and AGEs concentrations. Positive correlations were found between the gestational age at sampling and umbilical cord blood TAC and AGEs concentrations (TAC: rho = 0.26; p = 0.001; AGEs: rho = 0.35; p < 0.0001). There was no association between umbilical cord blood TAC, FRAP, TBARS and AGEs concentrations and selected aspects of short-term neonatal morbidity. CONCLUSIONS: Oxidative stress is associated with PPROM, as indicated by the presence of markers tested in the umbilical cord blood; however, the evaluated oxidative stress markers are not influenced by the presence of MIAC and/or HCA, and funisitis or subsequent development of selected aspects of short-term neonatal morbidity.
Entities:
Keywords:
Advanced glycation end products; ferric reducing antioxidant power; histological chorioamnionitis; microbial invasion of the amniotic cavity; preterm delivery; rupture of membranes; thiobarbituric acid-reacting substances; total antioxidant capacity
Authors: Ivana Musilova; Lenka Pliskova; Romana Gerychova; Petr Janku; Ondrej Simetka; Petr Matlak; Bo Jacobsson; Marian Kacerovsky Journal: PLoS One Date: 2017-12-12 Impact factor: 3.240
Authors: Silvia Martini; Laura Castellini; Roberta Parladori; Vittoria Paoletti; Arianna Aceti; Luigi Corvaglia Journal: Antioxidants (Basel) Date: 2021-12-18