| Literature DB >> 26304164 |
Xiaojun Qiu1, Xiaojuan He2, Qingfeng Huang1, Xianchen Liu1, Guan Sun1, Jun Guo1, Damin Yuan2, Lixiang Yang2, Na Ban2, Shaochen Fan2, Tao Tao3, Donglin Wang4.
Abstract
Overexpression of chaperonin containing t-complex polypeptide 1 (TCP1), or CCT, has been reported in various classes of malignancies. However, little is known about the expression of t-complex protein subunits TCP1theta (CCT8) in gliomas. In this study, the expression of CCT8 protein was detected using blotting analysis and immunohistochemistry. CCT8 was found to be overexpressed in gliomas and to correlate with the WHO grade of gliomas. To further investigate the biological function of CCT8 in gliomas, CCT8-silenced U87 glioblastoma multiforme (GBM) and U251MG cells were constructed using a small interference RNA (siRNA) sequence. The knockdown effect of CCT8 on proliferation and invasion in these cells was analyzed using the CCK8, flow cytometry cycle, scratch, transwell invasion and fluorescence assays. Compared with the controls, the glioma cells expressing CCT8-siRNA exhibited a significantly decreased proliferation and invasion capacity, as well as a dysregulated cell cytoskeleton. This study showed that high CCT8 protein expression might be related to poor outcome of glioma, and that CCT8 regulates the proliferation and invasion of glioblastomas.Entities:
Keywords: CCT8; Glioma; Invasion; Proliferation
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Year: 2015 PMID： 26304164 DOI： 10.1016/j.prp.2015.04.012
Source DB: PubMed Journal: Pathol Res Pract ISSN： 0344-0338 Impact factor: 3.250