BACKGROUND/AIMS: Lithium chloride (LiCl) has long been used as a psychiatric medication; however, its role in the differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) remains largely unknown. The aim of this study is to explore the effect of LiCl on the differentiation of BMSCs. METHODS: The roles of LiCl in osteogenic and adipogenic processes were observed using alizarin red staining and oil red O staining, respectively. The effects of LiCl on the Wnt and Hedgehog (Hh) pathways were investigated. RESULTS: Our data showed that LiCl effectively promoted osteogenesis and inhibited adipogenesis by simultaneously affecting the Wnt and Hh pathways. CONCLUSION: These results suggest that LiCl influences the differentiation of BMSCs directly through the Wnt and Hh pathways and thus may be a candidate drug for the treatment of osteoporosis.
BACKGROUND/AIMS: Lithium chloride (LiCl) has long been used as a psychiatric medication; however, its role in the differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) remains largely unknown. The aim of this study is to explore the effect of LiCl on the differentiation of BMSCs. METHODS: The roles of LiCl in osteogenic and adipogenic processes were observed using alizarin red staining and oil red O staining, respectively. The effects of LiCl on the Wnt and Hedgehog (Hh) pathways were investigated. RESULTS: Our data showed that LiCl effectively promoted osteogenesis and inhibited adipogenesis by simultaneously affecting the Wnt and Hh pathways. CONCLUSION: These results suggest that LiCl influences the differentiation of BMSCs directly through the Wnt and Hh pathways and thus may be a candidate drug for the treatment of osteoporosis.
Authors: L Zhao; G Ji; X Le; Z Luo; C Wang; M Feng; L Xu; Y Zhang; W B Lau; B Lau; Y Yang; L Lei; H Yang; Y Xuan; Y Chen; X Deng; T Yi; S Yao; X Zhao; Y Wei; S Zhou Journal: Oncogene Date: 2017-01-23 Impact factor: 9.867
Authors: Luis A Haro Durand; Gabriela E Vargas; Rosa Vera-Mesones; Alberto Baldi; María P Zago; María A Fanovich; Aldo R Boccaccini; Alejandro Gorustovich Journal: Materials (Basel) Date: 2017-07-03 Impact factor: 3.623
Authors: Kristina Glenske; Phil Donkiewicz; Alexander Köwitsch; Nada Milosevic-Oljaca; Patrick Rider; Sven Rofall; Jörg Franke; Ole Jung; Ralf Smeets; Reinhard Schnettler; Sabine Wenisch; Mike Barbeck Journal: Int J Mol Sci Date: 2018-03-12 Impact factor: 5.923