Luca Morandi1, Davide Gissi2, Achille Tarsitano3, Sofia Asioli1, Valentina Monti1, Giacomo Del Corso4, Claudio Marchetti4, Lucio Montebugnoli2, Maria Pia Foschini1. 1. Department of Biomedical and Neuro-Muscular Sciences, Section of Anatomic Pathology "M.Malpighi" at Bellaria Hospital (Head: Prof. Maria Pia Foschini, MD), University of Bologna, Italy. 2. Department of Biomedical and Neuro-Muscular Sciences, Section of Oral Science (Head: Prof. Lucio Montebugnoli, DDS), University of Bologna, Bologna, Italy. 3. Department of Biomedical and Neuro-Muscular Sciences, Section of Maxillofacial Surgery (Head: Prof. Claudio Marchetti, MD), University of Bologna, Italy. Electronic address: achille.tarsitano2@unibo.it. 4. Department of Biomedical and Neuro-Muscular Sciences, Section of Maxillofacial Surgery (Head: Prof. Claudio Marchetti, MD), University of Bologna, Italy.
Abstract
PURPOSE: Oral squamous cell carcinoma (OSCC) is commonly preceded by oral potentially malignant lesions (OPML). The aim of the present study was to assess, by bisulfite next-generation sequencing (NGS), the methylation status of a list of candidate genes obtained from oral brushings to early detect OPML and OSCC. MATERIAL AND METHODS: Oral brushing specimens from 11 OSCC, 11 high-grade squamous intraepithelial lesions (HG-SIL), 9 low-grade SIL (LG-SIL), 9 oral lichen planus (OLP), and 8 healthy donors were included in this study. We investigated, by means of bisulfite NGS, the promoter of GP1BB, ZAP70, KIF1A, p16[CDKN2A], CDH1, miR137, and miR375. Statistical significance between lesions and a pool of healthy donors were evaluated with the Mann-Whitney U test. RESULTS: ZAP70 was found to be hypermethylated in 100% of OSCC and HG-SIL and in 28.6% of LG-SIL. GP1BB hypomethylation was detected in 90.9% OSCC and HG-SIL and in 37.5% of LG-SIL. MiR137 was hypermethylated in 100% of OLP, 44.4% of OSCC, 40% HG-SIL, and 25% LG-SIL. KIF1A hypermethylation was found to be associated with TP53 mutations (p < 0.0001). CONCLUSION: In the present preliminary cohort of patients, DNA methylation analysis of GP1BB and ZAP70 seems to be a promising noninvasive tool for early detection of OSCC and HG SIL from oral brushing specimens.
PURPOSE:Oral squamous cell carcinoma (OSCC) is commonly preceded by oral potentially malignant lesions (OPML). The aim of the present study was to assess, by bisulfite next-generation sequencing (NGS), the methylation status of a list of candidate genes obtained from oral brushings to early detect OPML and OSCC. MATERIAL AND METHODS: Oral brushing specimens from 11 OSCC, 11 high-grade squamous intraepithelial lesions (HG-SIL), 9 low-grade SIL (LG-SIL), 9 oral lichen planus (OLP), and 8 healthy donors were included in this study. We investigated, by means of bisulfite NGS, the promoter of GP1BB, ZAP70, KIF1A, p16[CDKN2A], CDH1, miR137, and miR375. Statistical significance between lesions and a pool of healthy donors were evaluated with the Mann-Whitney U test. RESULTS:ZAP70 was found to be hypermethylated in 100% of OSCC and HG-SIL and in 28.6% of LG-SIL. GP1BB hypomethylation was detected in 90.9% OSCC and HG-SIL and in 37.5% of LG-SIL. MiR137 was hypermethylated in 100% of OLP, 44.4% of OSCC, 40% HG-SIL, and 25% LG-SIL. KIF1A hypermethylation was found to be associated with TP53 mutations (p < 0.0001). CONCLUSION: In the present preliminary cohort of patients, DNA methylation analysis of GP1BB and ZAP70 seems to be a promising noninvasive tool for early detection of OSCC and HG SIL from oral brushing specimens.
Authors: Davide B Gissi; Luca Morandi; Andrea Gabusi; Achille Tarsitano; Claudio Marchetti; Francesca Cura; Annalisa Palmieri; Lucio Montebugnoli; Sofia Asioli; Maria P Foschini; Luca Scapoli Journal: Int J Mol Sci Date: 2018-06-16 Impact factor: 5.923
Authors: Christopher G Németh; Christoph Röcken; Reiner Siebert; Jörg Wiltfang; Ole Ammerpohl; Volker Gassling Journal: PLoS One Date: 2019-04-09 Impact factor: 3.240
Authors: Davide B Gissi; Achille Tarsitano; Andrea Gabusi; Roberto Rossi; Giuseppe Attardo; Jacopo Lenzi; Claudio Marchetti; Lucio Montebugnoli; Maria P Foschini; Luca Morandi Journal: J Clin Med Date: 2019-12-02 Impact factor: 4.241
Authors: Julianna K Bronk; Chiraag Kapadia; Xiaogang Wu; Bhavana V Chapman; Rui Wang; Tatiana V Karpinets; Xingzhi Song; Andrew M Futreal; Jianhua Zhang; Ann H Klopp; Lauren E Colbert Journal: PLoS One Date: 2022-10-06 Impact factor: 3.752
Authors: Davide B Gissi; Viscardo P Fabbri; Andrea Gabusi; Jacopo Lenzi; Luca Morandi; Sofia Melotti; Sofia Asioli; Achille Tarsitano; Tiziana Balbi; Claudio Marchetti; Lucio Montebugnoli Journal: Int J Mol Sci Date: 2020-09-14 Impact factor: 5.923