| Literature DB >> 26301715 |
Christian Theilacker, Ann-Kristin Diederich, Andreas Otto, Irina G Sava, Dominique Wobser, Yinyin Bao, Katrin Hese, Melanie Broszat, Philipp Henneke, Dörte Becher, Johannes Huebner.
Abstract
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Year: 2015 PMID: 26301715 PMCID: PMC4547795 DOI: 10.1371/journal.pone.0136806
Source DB: PubMed Journal: PLoS One ISSN: 1932-6203 Impact factor: 3.240
Fig 3Stimulation of TNF-α production in RAW 264.7 mouse macrophages by lipoprotein-enriched cell membrane fractions of E. faecalis wild type and ΔbgsA.
RAW 264.7 cells were incubated with lipoprotein-enriched Triton X-114 extracts from total membrane protein fractions derived from the indicated E. faecalis strains. The concentration of lipoprotein extracts was measured photometrically and normalized to a bacterial cfu:RAW 264.7 cell ratio of 10,000:1. At 16 h, supernatants were collected and TNF-α concentrations were quantified by ELISA. LPS at a concentration of 100 ng/ml was used as positive control. Data represent mean ± SEM of triplicates. * p < 0.001 12030 ΔbgsA versus 12030 WT.