Literature DB >> 26301715

Correction: Enterococcus faecalis Glycolipids Modulate Lipoprotein-Content of the Bacterial Cell Membrane and Host Immune Response.

Christian Theilacker, Ann-Kristin Diederich, Andreas Otto, Irina G Sava, Dominique Wobser, Yinyin Bao, Katrin Hese, Melanie Broszat, Philipp Henneke, Dörte Becher, Johannes Huebner.   

Abstract

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Year:  2015        PMID: 26301715      PMCID: PMC4547795          DOI: 10.1371/journal.pone.0136806

Source DB:  PubMed          Journal:  PLoS One        ISSN: 1932-6203            Impact factor:   3.240


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There is an error in Fig 3. Fig. 2A has been incorrectly repeated. Please view the complete, correct Fig 3 here.
Fig 3

Stimulation of TNF-α production in RAW 264.7 mouse macrophages by lipoprotein-enriched cell membrane fractions of E. faecalis wild type and ΔbgsA.

RAW 264.7 cells were incubated with lipoprotein-enriched Triton X-114 extracts from total membrane protein fractions derived from the indicated E. faecalis strains. The concentration of lipoprotein extracts was measured photometrically and normalized to a bacterial cfu:RAW 264.7 cell ratio of 10,000:1. At 16 h, supernatants were collected and TNF-α concentrations were quantified by ELISA. LPS at a concentration of 100 ng/ml was used as positive control. Data represent mean ± SEM of triplicates. * p < 0.001 12030 ΔbgsA versus 12030 WT.

Stimulation of TNF-α production in RAW 264.7 mouse macrophages by lipoprotein-enriched cell membrane fractions of E. faecalis wild type and ΔbgsA.

RAW 264.7 cells were incubated with lipoprotein-enriched Triton X-114 extracts from total membrane protein fractions derived from the indicated E. faecalis strains. The concentration of lipoprotein extracts was measured photometrically and normalized to a bacterial cfu:RAW 264.7 cell ratio of 10,000:1. At 16 h, supernatants were collected and TNF-α concentrations were quantified by ELISA. LPS at a concentration of 100 ng/ml was used as positive control. Data represent mean ± SEM of triplicates. * p < 0.001 12030 ΔbgsA versus 12030 WT.
  1 in total

1.  Enterococcus faecalis Glycolipids Modulate Lipoprotein-Content of the Bacterial Cell Membrane and Host Immune Response.

Authors:  Christian Theilacker; Ann-Kristin Diederich; Andreas Otto; Irina G Sava; Dominique Wobser; Yinyin Bao; Katrin Hese; Melanie Broszat; Philipp Henneke; Dörte Becher; Johannes Huebner
Journal:  PLoS One       Date:  2015-07-14       Impact factor: 3.240

  1 in total

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