| Literature DB >> 26300544 |
Huihui Ji1, Yunliang Wang2, Guili Liu1, Xuting Xu1, Dongjun Dai1, Zhongming Chen3, Dongsheng Zhou3, Xiaohui Zhou4, Liyuan Han1, Ying Li5, Renjie Zhuo1, Qingxiao Hong1, Liting Jiang1, Xiaonan Zhang1, Yu Liu1, Lei Xu1, Lan Chang1, Jinfeng Li6, Pengyuan An1, Shiwei Duan7, Qinwen Wang8.
Abstract
As a member of the opioid family, κ-opioid receptors play important role in cognitive and learning functions. The purpose of this study was to evaluate the association of OPRK1 promoter methylation with Alzheimer's disease (AD). OPRK1 DNA methylation levels of 48 cases and 58 well matched controls were measured using the bisulphite pyrosequencing technology. Our results showed that there was a significant correlation between three CpG sites on the OPRK1 promoter region (r>=0.715, p<0.001). Thus, the mean methylation value of the three CpG sites was used for the case-control comparison. And our results showed there was a significantly higher OPRK1 promoter methylation in AD cases than in controls (p=0.006, adjusted p=0.012). Subsequent luciferase reporter assay showed the CpGs containing fragment of OPRK1 promoter significantly increased the expression of reporter gene (Fold=2.248, p=0.0235). In summary, our results suggested that OPRK1 promoter hypermethylation might increase the risk of AD through its regulation on the gene expression of OPRK1.Entities:
Keywords: Alzheimer disease; DNA methylation; Expression; OPRK1; Promoter
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Year: 2015 PMID： 26300544 DOI： 10.1016/j.neulet.2015.08.027
Source DB: PubMed Journal: Neurosci Lett ISSN： 0304-3940 Impact factor: 3.046