Literature DB >> 26294410

Intrathymic Injection.

Sugata Manna1, Avinash Bhandoola.   

Abstract

Intrathymic injection is used in several T cell-associated immunological studies to deliver cells or other substances directly into the thymus. Here, we describe the intrathymic injection procedure involving surgical incision of the mouse with or without a thoracotomy. Though this procedure can result in poor recovery, postsurgical complications, and distress to the animal, it is actually a simple procedure that can be carried out relatively easily and quickly with experience.

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Year:  2016        PMID: 26294410     DOI: 10.1007/978-1-4939-2809-5_17

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  5 in total

1.  Real Time In Vivo Tracking of Thymocytes in the Anterior Chamber of the Eye by Laser Scanning Microscopy.

Authors:  Elisa Oltra; Alejandro Caicedo
Journal:  J Vis Exp       Date:  2018-10-02       Impact factor: 1.355

2.  Preparation and Applications of Organotypic Thymic Slice Cultures.

Authors:  Aditi Sood; Mengqi Dong; Heather J Melichar
Journal:  J Vis Exp       Date:  2016-08-06       Impact factor: 1.355

3.  Intrathymic adeno-associated virus gene transfer rapidly restores thymic function and long-term persistence of gene-corrected T cells.

Authors:  Marie Pouzolles; Alice Machado; Mickaël Guilbaud; Magali Irla; Sarah Gailhac; Pierre Barennes; Daniela Cesana; Andrea Calabria; Fabrizio Benedicenti; Arnauld Sergé; Indu Raman; Quan-Zhen Li; Eugenio Montini; David Klatzmann; Oumeya Adjali; Naomi Taylor; Valérie S Zimmermann
Journal:  J Allergy Clin Immunol       Date:  2019-09-09       Impact factor: 10.793

4.  A Minimally Invasive, Accurate, and Efficient Technique for Intrathymic Injection in Mice.

Authors:  Michael T McGuire; Andrea Z Tuckett; Faith Myint; Johannes L Zakrzewski
Journal:  J Vis Exp       Date:  2022-08-23       Impact factor: 1.424

5.  Myc controls a distinct transcriptional program in fetal thymic epithelial cells that determines thymus growth.

Authors:  Jennifer E Cowan; Justin Malin; Yongge Zhao; Mina O Seedhom; Christelle Harly; Izumi Ohigashi; Michael Kelly; Yousuke Takahama; Jonathan W Yewdell; Maggie Cam; Avinash Bhandoola
Journal:  Nat Commun       Date:  2019-12-02       Impact factor: 14.919

  5 in total

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