| Literature DB >> 26294409 |
Levi J Rupp1, Liang Chen, Michael S Krangel, Craig H Bassing.
Abstract
PCR on genomic DNA isolated from lymphocyte populations is an invaluable technique to analyze T cell receptor (TCR) α and β gene rearrangements. Although this approach is powerful, it also has limitations that must be accounted for in experimental design and data interpretation. Here, we provide background required for understanding these limitations, and then outline standard PCR methods that can be used for analysis of TCRα and β gene rearrangements in mice.Entities:
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Year: 2016 PMID： 26294409 DOI： 10.1007/978-1-4939-2809-5_16
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745