| Literature DB >> 26294402 |
Francis A Flomerfelt1, Ronald E Gress.
Abstract
Radiation chimeras are prepared by subjecting recipient mice to sublethal or lethal dose of irradiation and injecting them with hematopoietic stem cells (HSC) from untreated donor mice. HSC can be obtained from bone marrow or fetal liver. This technique is a powerful tool when coupled with gene targeting strategies to investigate function of HSCs, thymocyte development, and T cell function. This protocol describes how to produce bone marrow or fetal liver chimeras.Entities:
Mesh：
Year: 2016 PMID： 26294402 PMCID： PMC7473463 DOI： 10.1007/978-1-4939-2809-5_9
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745