Natasha DeJarnett1, Ray Yeager1, Daniel J Conklin1, Jongmin Lee1, Timothy E O'Toole1, James McCracken1, Wes Abplanalp1, Sanjay Srivastava1, Daniel W Riggs1, Ihab Hamzeh1, Stephen Wagner1, Atul Chugh1, Andrew DeFilippis1, Tiffany Ciszewski1, Brad Wyatt1, Carrie Becher1, Deirdre Higdon1, Kenneth S Ramos1, David J Tollerud1, John A Myers1, Shesh N Rai1, Jasmit Shah1, Nagma Zafar1, Sathya S Krishnasamy1, Sumanth D Prabhu1, Aruni Bhatnagar2. 1. From the Diabetes and Obesity Center (N.D., R.Y., D.J.C., J.L., T.E.O., J.M., W.A., S.S., D.W.R., A.C., A.D., T.C., B.W., S.N.R., J.S., N.Z., S.S.K., A.B.), Department of Environmental and Occupational Health Sciences (N.D., R.Y., D.J.T.), Institute of Molecular Cardiology (N.D., D.J.C., J.L., T.E.O., J.M., W.A., S.S., D.W.R., S.W., A.C., A.D., T.C., B.W., C.B., D.H., J.S., S.D.P., A.B.), Department of Pediatrics (J.A.M.), Department of Bioinformatics and Biostatics (S.N.R.), Biostatistics Shared Facility, JG Brown Cancer Center (S.N.R.), Division of Endocrinology, Metabolism and Diabetes, Department of Medicine (S.S.K.), and Department of Biochemistry and Molecular Biology (K.S.R., A.B.), University of Louisville, KY; Division of Cardiology, Department of Medicine, Baylor College of Medicine, Houston, TX (I.H.); Department of Medicine, Johns Hopkins University, Baltimore, MD (A.D.); and Division of Cardiovascular Disease, Department of Medicine, University of Alabama at Birmingham (S.D.P.). 2. From the Diabetes and Obesity Center (N.D., R.Y., D.J.C., J.L., T.E.O., J.M., W.A., S.S., D.W.R., A.C., A.D., T.C., B.W., S.N.R., J.S., N.Z., S.S.K., A.B.), Department of Environmental and Occupational Health Sciences (N.D., R.Y., D.J.T.), Institute of Molecular Cardiology (N.D., D.J.C., J.L., T.E.O., J.M., W.A., S.S., D.W.R., S.W., A.C., A.D., T.C., B.W., C.B., D.H., J.S., S.D.P., A.B.), Department of Pediatrics (J.A.M.), Department of Bioinformatics and Biostatics (S.N.R.), Biostatistics Shared Facility, JG Brown Cancer Center (S.N.R.), Division of Endocrinology, Metabolism and Diabetes, Department of Medicine (S.S.K.), and Department of Biochemistry and Molecular Biology (K.S.R., A.B.), University of Louisville, KY; Division of Cardiology, Department of Medicine, Baylor College of Medicine, Houston, TX (I.H.); Department of Medicine, Johns Hopkins University, Baltimore, MD (A.D.); and Division of Cardiovascular Disease, Department of Medicine, University of Alabama at Birmingham (S.D.P.) aruni@louisville.edu.
Abstract
OBJECTIVES: Previous studies have shown that residential proximity to a roadway is associated with increased cardiovascular disease risk. Yet, the nature of this association remains unclear, and its effect on individual cardiovascular disease risk factors has not been assessed. The objective of this study was to determine whether residential proximity to roadways influences systemic inflammation and the levels of circulating angiogenic cells. APPROACH AND RESULTS: In a cross-sectional study, cardiovascular disease risk factors, blood levels of C-reactive protein, and 15 antigenically defined circulating angiogenic cell populations were measured in participants (n=316) with moderate-to-high cardiovascular disease risk. Attributes of roadways surrounding residential locations were assessed using geographic information systems. Associations between road proximity and cardiovascular indices were analyzed using generalized linear models. Close proximity (<50 m) to a major roadway was associated with lower income and higher rates of smoking but not C-reactive protein levels. After adjustment for potential confounders, the levels of circulating angiogenic cells in peripheral blood were significantly elevated in people living in close proximity to a major roadway (CD31(+)/AC133(+), AC133(+), CD34(+)/AC133(+), and CD34(+)/45(dim)/AC133(+) cells) and positively associated with road segment distance (CD31(+)/AC133(+), AC133(+), and CD34(+)/AC133(+) cells), traffic intensity (CD31(+)/AC133(+) and AC133(+) cells), and distance-weighted traffic intensity (CD31(+)/34(+)/45(+)/AC133(+) cells). CONCLUSIONS: Living close to a major roadway is associated with elevated levels of circulating cells positive for the early stem marker AC133(+). This may reflect an increased need for vascular repair. Levels of these cells in peripheral blood may be a sensitive index of cardiovascular injury because of residential proximity to roadways.
OBJECTIVES: Previous studies have shown that residential proximity to a roadway is associated with increased cardiovascular disease risk. Yet, the nature of this association remains unclear, and its effect on individual cardiovascular disease risk factors has not been assessed. The objective of this study was to determine whether residential proximity to roadways influences systemic inflammation and the levels of circulating angiogenic cells. APPROACH AND RESULTS: In a cross-sectional study, cardiovascular disease risk factors, blood levels of C-reactive protein, and 15 antigenically defined circulating angiogenic cell populations were measured in participants (n=316) with moderate-to-high cardiovascular disease risk. Attributes of roadways surrounding residential locations were assessed using geographic information systems. Associations between road proximity and cardiovascular indices were analyzed using generalized linear models. Close proximity (<50 m) to a major roadway was associated with lower income and higher rates of smoking but not C-reactive protein levels. After adjustment for potential confounders, the levels of circulating angiogenic cells in peripheral blood were significantly elevated in people living in close proximity to a major roadway (CD31(+)/AC133(+), AC133(+), CD34(+)/AC133(+), and CD34(+)/45(dim)/AC133(+) cells) and positively associated with road segment distance (CD31(+)/AC133(+), AC133(+), and CD34(+)/AC133(+) cells), traffic intensity (CD31(+)/AC133(+) and AC133(+) cells), and distance-weighted traffic intensity (CD31(+)/34(+)/45(+)/AC133(+) cells). CONCLUSIONS: Living close to a major roadway is associated with elevated levels of circulating cells positive for the early stem marker AC133(+). This may reflect an increased need for vascular repair. Levels of these cells in peripheral blood may be a sensitive index of cardiovascular injury because of residential proximity to roadways.
Authors: S Shintani; T Murohara; H Ikeda; T Ueno; T Honma; A Katoh; K Sasaki; T Shimada; Y Oike; T Imaizumi Journal: Circulation Date: 2001-06-12 Impact factor: 29.690
Authors: D Lyden; K Hattori; S Dias; C Costa; P Blaikie; L Butros; A Chadburn; B Heissig; W Marks; L Witte; Y Wu; D Hicklin; Z Zhu; N R Hackett; R G Crystal; M A Moore; K A Hajjar; K Manova; R Benezra; S Rafii Journal: Nat Med Date: 2001-11 Impact factor: 53.440
Authors: Tiffany M Powell; Jonathan D Paul; Jonathan M Hill; Michael Thompson; Moshe Benjamin; Maria Rodrigo; J Philip McCoy; Elizabeth J Read; Hanh M Khuu; Susan F Leitman; Toren Finkel; Richard O Cannon Journal: Arterioscler Thromb Vasc Biol Date: 2004-11-29 Impact factor: 8.311
Authors: C Arden Pope; Richard T Burnett; George D Thurston; Michael J Thun; Eugenia E Calle; Daniel Krewski; John J Godleski Journal: Circulation Date: 2003-12-15 Impact factor: 29.690
Authors: Mercedes Medina-Ramón; Robert Goldberg; Steven Melly; Murray A Mittleman; Joel Schwartz Journal: Environ Health Perspect Date: 2008-04 Impact factor: 9.031
Authors: Shahid P Baba; Deqing Zhang; Mahavir Singh; Sujith Dassanayaka; Zhengzhi Xie; Ganapathy Jagatheesan; Jingjing Zhao; Virginia K Schmidtke; Kenneth R Brittian; Michael L Merchant; Daniel J Conklin; Steven P Jones; Aruni Bhatnagar Journal: J Mol Cell Cardiol Date: 2018-04-05 Impact factor: 5.000
Authors: Igor N Zelko; Breandon S Taylor; Trinath P Das; Walter H Watson; Israel D Sithu; Banrida Wahlang; Marina V Malovichko; Matthew C Cave; Sanjay Srivastava Journal: Environ Toxicol Date: 2021-10-30 Impact factor: 4.109
Authors: Marina V Malovichko; Wesley T Abplanalp; Samantha A McFall; Breandon S Taylor; Nalinie S Wickramasinghe; Israel D Sithu; Igor N Zelko; Shizuka Uchida; Bradford G Hill; Saurin R Sutaria; Michael H Nantz; Aruni Bhatnagar; Daniel J Conklin; Timothy E O'Toole; Sanjay Srivastava Journal: Toxicol Appl Pharmacol Date: 2021-10-05 Impact factor: 4.460
Authors: Daniel J Conklin; Suzaynn Schick; Michael J Blaha; Alex Carll; Andrew DeFilippis; Peter Ganz; Michael E Hall; Naomi Hamburg; Tim O'Toole; Lindsay Reynolds; Sanjay Srivastava; Aruni Bhatnagar Journal: Am J Physiol Heart Circ Physiol Date: 2019-02-01 Impact factor: 4.733
Authors: Cavin K Ward-Caviness; William E Kraus; Colette Blach; Carol S Haynes; Elaine Dowdy; Marie Lynn Miranda; Robert Devlin; David Diaz-Sanchez; Wayne E Cascio; Shaibal Mukerjee; Casson Stallings; Luther A Smith; Simon G Gregory; Svati H Shah; Lucas M Neas; Elizabeth R Hauser Journal: Arterioscler Thromb Vasc Biol Date: 2017-11-30 Impact factor: 8.311
Authors: Bernd Niemann; Susanne Rohrbach; Mark R Miller; David E Newby; Valentin Fuster; Jason C Kovacic Journal: J Am Coll Cardiol Date: 2017-07-11 Impact factor: 24.094
Authors: Cavin K Ward-Caviness; Lucas M Neas; Colette Blach; Carol S Haynes; Karen LaRocque-Abramson; Elizabeth Grass; Elaine Dowdy; Robert B Devlin; David Diaz-Sanchez; Wayne E Cascio; Marie Lynn Miranda; Simon G Gregory; Svati H Shah; William E Kraus; Elizabeth R Hauser Journal: PLoS One Date: 2016-04-15 Impact factor: 3.240