Literature DB >> 26291430

A novel electrochemical immunosensor for the quantitative detection of 5-hydroxymethylcytosine in genomic DNA of breast cancer tissue.

Zhiqing Yang1, Wenjing Jiang, Fei Liu, Yunlei Zhou, Huanshun Yin, Shiyun Ai.   

Abstract

A novel electrochemical immunosensor was fabricated for the quantitative detection of 5-hydroxymethylcytosine (5-hmC) in genomic DNA based on anti-5-hmC antibody, biotin functionalized phos-tag and avidin functionalized alkaline phosphatase. It is demonstrated that the levels of 5-hmC are dramatically reduced in human breast cancer tissue compared with those in normal tissue.

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Year:  2015        PMID： 26291430     DOI： 10.1039/c5cc05921f

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  6 in total

Review 1.  Formation and repair of oxidatively generated damage in cellular DNA.

Authors:  Jean Cadet; Kelvin J A Davies; Marisa Hg Medeiros; Paolo Di Mascio; J Richard Wagner
Journal:  Free Radic Biol Med       Date:  2017-01-02       Impact factor: 7.376

2.  Photoelectrochemical assay for DNA hydroxymethylation determination based on the inhibited photoactivity of black TiO2 nanosphere by ZnO.

Authors:  Yan Chen; Huanshun Yin; Chengji Sui; Weizhang Fu; Yue Yang; Shiyun Ai
Journal:  Mikrochim Acta       Date:  2020-02-05       Impact factor: 5.833

3.  DNAzyme assisted recycling amplification method for ultrasensitive amperometric determination of lead(II) based on the use of a hairpin assembly on a composite prepared from nitrogen doped graphene, perylenetetracarboxylic anhydride, thionine and gold nanoparticles.

Authors:  Yidan Ma; Chao Yu; Yujie Yu; Jun Chen; Rufei Gao; Junlin He
Journal:  Mikrochim Acta       Date:  2019-09-11       Impact factor: 5.833

4.  Electrochemical affinity biosensors for fast detection of gene-specific methylations with no need for bisulfite and amplification treatments.

Authors:  Eloy Povedano; Eva Vargas; Víctor Ruiz-Valdepeñas Montiel; Rebeca M Torrente-Rodríguez; María Pedrero; Rodrigo Barderas; Pablo San Segundo-Acosta; Alberto Peláez-García; Marta Mendiola; David Hardisson; Susana Campuzano; José M Pingarrón
Journal:  Sci Rep       Date:  2018-04-23       Impact factor: 4.379

5.  Integrated Microfluidic Devices Fabricated in Poly (Methyl Methacrylate) (PMMA) for On-site Therapeutic Drug Monitoring of Aminoglycosides in Whole Blood.

Authors:  Zaidon T Al-Aqbi; Yiing C Yap; Feng Li; Michael C Breadmore
Journal:  Biosensors (Basel)       Date:  2019-01-30

6.  Magnetic Immunosensor Coupled to Enzymatic Signal for Determination of Genomic DNA Methylation.

Authors:  Yitao Liang; Bin Zhang; Zexin Xue; Xuesong Ye; Bo Liang
Journal:  Biosensors (Basel)       Date:  2022-03-04
  6 in total

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