| Literature DB >> 26291147 |
Jonatas Campos Almeida1, Felippe Danyel Cardoso Martins1, José Maurício Ferreira Neto1, Maíra Moreira Dos Santos1, João Luis Garcia1, Italmar Teodorico Navarro1, Emília Kiyomi Kuroda2, Roberta Lemos Freire1.
Abstract
The purpose of this study was to investigate the occurrence of Cryptosporidium spp. and Giardia spp. in a public water-treatment system. Samples of raw and treated water were collected and concentrated using the membrane filtration technique. Direct Immunofluorescence Test was performed on the samples. DNA extraction using a commercial kit was performed and the DNA extracted was submitted to a nested-PCR reaction (n-PCR) and sequencing. In the immunofluorescence, 2/24 (8.33%) samples of raw water were positive for Giardia spp.. In n-PCR and sequencing, 2/24 (8.33%) samples of raw water were positive for Giardia spp., and 2/24 (8.33%) samples were positive for Cryptosporidium spp.. The sequencing showed Cryptosporidium parvum and Giardia duodenalis DNA. In raw water, there was moderate correlation among turbidity, color and Cryptosporidium spp. and between turbidity and Giardia spp.. The presence of these protozoans in the water indicates the need for monitoring for water-treatment companies.Entities:
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Year: 2015 PMID： 26291147 DOI： 10.1590/S1984-29612015051
Source DB: PubMed Journal: Rev Bras Parasitol Vet ISSN： 0103-846X