Jie Du1, Yunzi Chen, Yongyan Shi, Tianjing Liu, Yong Cao, Yue Tang, Xin Ge, Hongguang Nie, Changqing Zheng, Yan Chun Li. 1. *Institute of Metabolic Disease Research and Drug Development, China Medical University, Shenyang, China; †Department of Medicine, Division of Biological Sciences, The University of Chicago, Chicago, Illinois; ‡Division of Neonatology, Shengjing Hospital, China Medical University, Shenyang, China; and §Division of Gastroenterology, Shengjing Hospital, China Medical University, Shenyang, China.
Abstract
BACKGROUND: The myosin light chain kinase (MLCK) pathway controls intestinal epithelial barrier permeability by regulating the tight junction. 1,25-dihydroxyvitamin D (1,25(OH)2D3)-vitamin D receptor (VDR) signaling protects the epithelial barrier, but the molecular mechanism is incompletely understood. METHODS: MLCK activation and barrier permeability were studied using monolayers of HCT116, Caco-2, and SW480 cells treated with tissue necrosis factor α with or without 1,25(OH)2D3. The MLCK pathway was analyzed in normal and inflamed colonic biopsies from patients with ulcerative colitis. Colonic mucosal barrier permeability and MLCK activation were also investigated using trinitrobenzene sulfonic acid-induced colitis models in vitamin D analog paricalcitol-treated wild-type mice and mice carrying VDR deletion in colonic epithelial cells. RESULTS: Tissue necrosis factor α increased cell monolayer permeability and induced long isoform of MLCK expression and myosin II regulatory light chain (MLC) phosphorylation, and 1,25(OH)2D3 blocked tissue necrosis factor α-induced increases in monolayer permeability and MLCK-MLC pathway activation by a VDR-dependent fashion. 1,25(OH)2D3 directly suppressed long MLCK expression by attenuating NF-κB activation, and chromatin immunoprecipitation assays confirmed that 1,25(OH)2D3 disrupted p65 binding to 3 κB sites in long MLCK gene promoter. In human ulcerative colitis biopsies, VDR reduction was associated with increases in long MLCK expression and MLC phosphorylation. In trinitrobenzene sulfonic acid colitis models, paricalcitol ameliorated colitis, attenuated the increase in mucosal barrier permeability, and inhibited long MLCK induction and MLC phosphorylation. In contrast, mice with colonic epithelial VDR deletion exhibited more robust increases in mucosal barrier permeability and MLCK activation compared with wild-type mice. CONCLUSIONS: These data demonstrate that 1,25(OH)2D3-VDR signaling preserves the mucosal barrier integrity by abrogating MLCK-dependent tight junction dysregulation during colonic inflammation.
BACKGROUND: The myosin light chain kinase (MLCK) pathway controls intestinal epithelial barrier permeability by regulating the tight junction. 1,25-dihydroxyvitamin D (1,25(OH)2D3)-vitamin D receptor (VDR) signaling protects the epithelial barrier, but the molecular mechanism is incompletely understood. METHODS:MLCK activation and barrier permeability were studied using monolayers of HCT116, Caco-2, and SW480 cells treated with tissue necrosis factor α with or without 1,25(OH)2D3. The MLCK pathway was analyzed in normal and inflamed colonic biopsies from patients with ulcerative colitis. Colonic mucosal barrier permeability and MLCK activation were also investigated using trinitrobenzene sulfonic acid-induced colitis models in vitamin D analog paricalcitol-treated wild-type mice and mice carrying VDR deletion in colonic epithelial cells. RESULTS: Tissue necrosis factor α increased cell monolayer permeability and induced long isoform of MLCK expression and myosin II regulatory light chain (MLC) phosphorylation, and 1,25(OH)2D3 blocked tissue necrosis factor α-induced increases in monolayer permeability and MLCK-MLC pathway activation by a VDR-dependent fashion. 1,25(OH)2D3 directly suppressed long MLCK expression by attenuating NF-κB activation, and chromatin immunoprecipitation assays confirmed that 1,25(OH)2D3 disrupted p65 binding to 3 κB sites in long MLCK gene promoter. In humanulcerative colitis biopsies, VDR reduction was associated with increases in long MLCK expression and MLC phosphorylation. In trinitrobenzene sulfonic acidcolitis models, paricalcitol ameliorated colitis, attenuated the increase in mucosal barrier permeability, and inhibited long MLCK induction and MLC phosphorylation. In contrast, mice with colonic epithelialVDR deletion exhibited more robust increases in mucosal barrier permeability and MLCK activation compared with wild-type mice. CONCLUSIONS: These data demonstrate that 1,25(OH)2D3-VDR signaling preserves the mucosal barrier integrity by abrogating MLCK-dependent tight junction dysregulation during colonic inflammation.
Authors: M R Haussler; G K Whitfield; C A Haussler; J C Hsieh; P D Thompson; S H Selznick; C E Dominguez; P W Jurutka Journal: J Bone Miner Res Date: 1998-03 Impact factor: 6.741
Authors: Daniel R Clayburgh; Shari Rosen; Edwina D Witkowski; Fengjun Wang; Stephanie Blair; Steven Dudek; Joe G N Garcia; John C Alverdy; Jerrold R Turner Journal: J Biol Chem Date: 2004-10-26 Impact factor: 5.157
Authors: Lei He; Tianjing Liu; Yongyan Shi; Feng Tian; Huiyuan Hu; Dilip K Deb; Yinyin Chen; Marc Bissonnette; Yan Chun Li Journal: Endocrinology Date: 2018-02-01 Impact factor: 4.736
Authors: C Adam Lorentz; Zhe Liang; Mei Meng; Ching-Wen Chen; Benyam P Yoseph; Elise R Breed; Rohit Mittal; Nathan J Klingensmith; Alton B Farris; Eileen M Burd; Michael Koval; Mandy L Ford; Craig M Coopersmith Journal: Mol Med Date: 2017-06-07 Impact factor: 6.354