| Literature DB >> 26283021 |
Adam Hedberg-Buenz1, Mark A Christopher2, Carly J Lewis3, Kacie J Meyer3, Danielle S Rudd4, Laura M Dutca5, Kai Wang6, Mona K Garvin7, Todd E Scheetz8, Michael D Abràmoff9, Matthew M Harper5, Michael G Anderson10.
Abstract
The present article introduces RetFM-J, a semi-automated ImageJ-based module that detects, counts, and collects quantitative data on nuclei of the inner retina from H&E-stained whole-mounted retinas. To illustrate performance, computer-derived outputs were analyzed in inbred C57BL/6J mice. Automated characterization yielded computer-derived outputs that closely matched manual counts. As a method using open-source software that is freely available, inexpensive staining reagents that are robust, and imaging equipment that is routine to most laboratories, RetFM-J could be utilized in a wide variety of experiments benefiting from high-throughput, quantitative, uniform analyses of total cellularity in the inner retina. Published by Elsevier Ltd.Entities:
Keywords: Cell counter; Image analysis; ImageJ; Quantitative analysis; Retinal ganglion cell layer; Retinal whole-mount
Mesh：
Year: 2015 PMID： 26283021 PMCID： PMC4753132 DOI： 10.1016/j.exer.2015.07.020
Source DB: PubMed Journal: Exp Eye Res ISSN： 0014-4835 Impact factor: 3.467