Karl Ottmann1, Michael Tronnier1, Christina Mitteldorf2. 1. Department of Dermatology, Venereology, and Allergology, HELIOS-Klinikum Hildesheim, Hildesheim, Germany. 2. Department of Dermatology, Venereology, and Allergology, HELIOS-Klinikum Hildesheim, Hildesheim, Germany. Electronic address: christina.mitteldorf@helios-kliniken.de.
Abstract
BACKGROUND: The mitotic rate is an important prognostic criterion in patients with thin melanoma ≤ 1 mm. OBJECTIVE: The aim of this study was to investigate the reproducibility of the mitotic rate in thin melanoma in hematoxylin-eosin (H&E) stain and compare it with the detection of mitotic figures by immunohistochemistry. METHODS: The number of mitoses stated in the routine diagnostic report in 190 pT1 melanomas was compared with the number gained from re-evaluation of H&E sections and the number detected after staining with the mitotic marker, phosphohistone H3 (PHH3). Two different approaches were used for choosing the "hot spot" for evaluation (dermal vs epidermal/dermal). RESULTS: Comparing routine H&E-stained slides with re-evaluation slides, the number of mitotic figures was slightly variable. However, findings did not result in a change of the tumor stage. In 34% of the tumors with dermal mitotic figures on H&E, mitoses could not be found in the corresponding PHH3 slide anymore. In 4% of the cases, stage relevant mitoses could only be found by PHH3 immunohistochemistry. LIMITATION: This is a single center study. CONCLUSION: Immunohistochemical staining for mitotic figures does not replace a careful evaluation of H&E-stained slides. Immunohistochemical detection of mitosis is only an additional tool; the time-saving effect is therefore negligible.
BACKGROUND: The mitotic rate is an important prognostic criterion in patients with thin melanoma ≤ 1 mm. OBJECTIVE: The aim of this study was to investigate the reproducibility of the mitotic rate in thin melanoma in hematoxylin-eosin (H&E) stain and compare it with the detection of mitotic figures by immunohistochemistry. METHODS: The number of mitoses stated in the routine diagnostic report in 190 pT1 melanomas was compared with the number gained from re-evaluation of H&E sections and the number detected after staining with the mitotic marker, phosphohistone H3 (PHH3). Two different approaches were used for choosing the "hot spot" for evaluation (dermal vs epidermal/dermal). RESULTS: Comparing routine H&E-stained slides with re-evaluation slides, the number of mitotic figures was slightly variable. However, findings did not result in a change of the tumor stage. In 34% of the tumors with dermal mitotic figures on H&E, mitoses could not be found in the corresponding PHH3 slide anymore. In 4% of the cases, stage relevant mitoses could only be found by PHH3 immunohistochemistry. LIMITATION: This is a single center study. CONCLUSION: Immunohistochemical staining for mitotic figures does not replace a careful evaluation of H&E-stained slides. Immunohistochemical detection of mitosis is only an additional tool; the time-saving effect is therefore negligible.
Authors: A Piñero-Madrona; G Ruiz-Merino; P Cerezuela Fuentes; E Martínez-Barba; J N Rodríguez-López; J Cabezas-Herrera Journal: Clin Transl Oncol Date: 2019-02-19 Impact factor: 3.405
Authors: Min Jeong Kim; Mi Jung Kwon; Ho Suk Kang; Kyung Chan Choi; Eun Sook Nam; Seong Jin Cho; Hye-Rim Park; Soo Kee Min; Jinwon Seo; Ji-Young Choe; Hyoung-Chul Park Journal: Biomed Res Int Date: 2018-03-27 Impact factor: 3.411