Ali Berraaouan1, Ziyyat Abderrahim1, Mekhfi Hassane1, Legssyer Abdelkhaleq1, Aziz Mohammed1, Bnouham Mohamed2. 1. Laboratory of Physiology and Ethnopharmacology-URAC40, Mohammed First University, Faculty of Sciences, Oujda, Morocco. 2. Laboratory of Physiology and Ethnopharmacology-URAC40, Mohammed First University, Faculty of Sciences, Oujda, Morocco. Electronic address: mbnouham@yahoo.fr.
Abstract
OBJECTIVE: To evaluate the in vitro antioxidant power of cactus pear seed oil [Opuntia ficus-indica L. MILL. (CPSO)] and its protective effect against chemically induced diabetes mellitus in mice. METHODS: The in vitro antioxidant effect of CPSO was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. The preventive effect was conducted on Swiss albino mice treated with CPSO (2 mL/kg, per os), before and after a single intraperitoneal alloxan administration (100 mg/kg). Survival rate, body weight and fasting blood glucose were measured and histopathological analysis of pancreas was performed to evaluate alloxan-induced tissue injuries. RESULTS: CPSO exhibited an antioxidant effect in DPPH scavenging assay. Moreover, the administration of CPSO (2 mL/kg) significantly attenuated alloxan-induced death and hyperglycemia (P < 0.001) in treated mice. Morphometric study of pancreas revealed that CPSO significantly protected islets of langerhans against alloxan induced-tissue alterations. CONCLUSIONS: Based on theses results, CPSO can prevente alloxan-induced-diabetes by quenching free radicals produced by alloxan and inhibiting tissue injuries in pancreatic β-cells.
OBJECTIVE: To evaluate the in vitro antioxidant power of cactus pearseed oil [Opuntia ficus-indica L. MILL. (CPSO)] and its protective effect against chemically induced diabetes mellitus in mice. METHODS: The in vitro antioxidant effect of CPSO was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. The preventive effect was conducted on Swiss albino mice treated with CPSO (2 mL/kg, per os), before and after a single intraperitoneal alloxan administration (100 mg/kg). Survival rate, body weight and fasting blood glucose were measured and histopathological analysis of pancreas was performed to evaluate alloxan-induced tissue injuries. RESULTS:CPSO exhibited an antioxidant effect in DPPH scavenging assay. Moreover, the administration of CPSO (2 mL/kg) significantly attenuated alloxan-induced death and hyperglycemia (P < 0.001) in treated mice. Morphometric study of pancreas revealed that CPSO significantly protected islets of langerhans against alloxan induced-tissue alterations. CONCLUSIONS: Based on theses results, CPSO can prevente alloxan-induced-diabetes by quenching free radicals produced by alloxan and inhibiting tissue injuries in pancreatic β-cells.
Authors: Mohamed Bouhrim; Hayat Ouassou; Salima Boutahiri; Nour Elhouda Daoudi; Hamza Mechchate; Bernard Gressier; Bruno Eto; Hamada Imtara; Amal A Alotaibi; Mohammed Al-Zharani; Abderrahim Ziyyat; Hassane Mekhfi; Abdelkhaleq Legssyer; Mohammed Aziz; Mohamed Bnouham Journal: Molecules Date: 2021-03-17 Impact factor: 4.411
Authors: María Del Socorro Santos Díaz; Ana-Paulina Barba de la Rosa; Cécile Héliès-Toussaint; Françoise Guéraud; Anne Nègre-Salvayre Journal: Oxid Med Cell Longev Date: 2017-04-09 Impact factor: 6.543
Authors: Sana Bardaa; Khouloud Makni; Ons Boudaouara; Tarek Bardaa; Naourez Ktari; Selim Hachicha; Riadh Ben Salah; Rim Kallel; Zouheir Sahnoun; Sami Boufi Journal: Biomed Res Int Date: 2021-10-13 Impact factor: 3.411