| Literature DB >> 26267088 |
Luke P Randall1, Fabrizio Lemma2, Mark Koylass2, Jon Rogers2, Roger D Ayling2, Danny Worth2, Monika Klita2, Andrew Steventon2, Kirsty Line2, Peter Wragg2, Jakub Muchowski2, Markus Kostrzewa3, Adrian M Whatmore2.
Abstract
Matrix-Assisted Laser Desorption Ionisation-Time of Flight (MALDI-ToF) Mass Spectrometry with Bruker MALDI Biotyper software was evaluated as a method for identifying veterinary bacteria. For 620 isolates (~100 bacterial species), identification by MALDI-ToF and non-16S rDNA methods (mainly phenotypic/biochemical) agreed to species-level (95.3%) and to species/genus-level (100%), but in the absence of 16S rDNA as a gold standard. For a further panel of 107 anaerobes and 234 aerobes (~100 bacteria species) using 16S rDNA results as the gold standard, MALDI-ToF/biochemical tests showed 97.8/96.6% species-level and 99.6/93.5% genus-level agreement for aerobes and 95.3/93.6% species-level and 100/95.3% genus-level agreement for anaerobes compared to the gold standard. Where results were obtained from direct spots, direct spots overlaid with formic acid and extracts, 89.4% of 180 aerobes and 90.1% of 152 anaerobes were identified by MALDI-ToF. MALDI-ToF was shown to be a rapid and reliable method to identify veterinary bacteria. CrownEntities:
Keywords: 16S rDNA; Bacterial identification; Biochemical; MALDI-ToF
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Year: 2015 PMID： 26267088 DOI： 10.1016/j.rvsc.2015.05.018
Source DB: PubMed Journal: Res Vet Sci ISSN： 0034-5288 Impact factor: 2.534